Methods for producing probes capable of distinguishing DNA from

Details Methods for producing probes capable of distinguishing DNA from Methods for producing probes capable of distinguishing DNA from

1998-07-14

2000-12-12

Campbell, Eggerton A.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing compound containing saccharide radical

435 6, C12P 1934, C12Q 168

Patent

active

061597139

ABSTRACT:
Methodology is provided for developing probes for identifying sequence differences between two related DNA populations, sets of DNA fragments or collections of restriction-endonuclease-cleaved DNA or cDNA. The method employs an initial stage to obtain a representation of both DNA populations, namely using the PCR to produce relatively short fragments, referred to as amplicons. Tester amplicons containing target DNA, sequences of interest, are ligated to adaptors and mixed with excess driver amplicons under melting and annealing conditions, followed by PCR amplification. The process may be repeated so as to greatly enrich the target DNA. Optionally, the target DNA may then be cloned and the DNA used as probes.

REFERENCES:
patent: 5436142 (1995-07-01), Wigler et al.
patent: 5501964 (1996-03-01), Wigler et al.

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