Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Using tissue cell culture to make a protein or polypeptide
Reexamination Certificate
2008-03-18
2008-03-18
Benzion, Gary (Department: 1637)
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Using tissue cell culture to make a protein or polypeptide
C435S091100
Reexamination Certificate
active
10899139
ABSTRACT:
The present invention is directed to compositions and methods for enhancing synthesis of nucleic acid molecules, particularly GC-rich nucleic acid molecules. Specifically, the invention provides compositions comprising one or more nitrogen-containing organic compounds having a formula selected from the group consisting of formula I and formula II (or salts or derivatives thereof), preferably 4-methylmorpholine N-oxide or betaine (carboxymethyltrimethylammonium), and further comprising one or more compounds selected from the group consisting of proline and an N-alkylimidazole compound, and more preferably proline, 1-methylimidazole or 4-methylimidazole. The invention further relates to methods for enhanced, high-fidelity synthesis of nucleic acid molecules, including via amplification (particularly PCR), reverse transcription, and sequencing methods. The invention also relates to nucleic acid molecules synthesized by these methods, to fragments or derivatives thereof, and to vectors and host cells comprising such nucleic acid molecules, fragments, or derivatives. The invention also relates to kits for synthesizing, amplifying, reverse transcribing or sequencing nucleic acid molecules comprising one or more of the compositions of the invention.
REFERENCES:
patent: 4683202 (1987-07-01), Mullis
patent: 4767699 (1988-08-01), Vary et al.
patent: 4800159 (1989-01-01), Mullis et al.
patent: 5165925 (1992-11-01), Leong
patent: 5187085 (1993-02-01), Lee
patent: 5350672 (1994-09-01), Oberst et al.
patent: 5484702 (1996-01-01), Ludwig
patent: 5500356 (1996-03-01), Li et al.
patent: 5545540 (1996-08-01), Mian
patent: 5759778 (1998-06-01), Li et al.
patent: 5871929 (1999-02-01), Barnes
patent: 6013488 (2000-01-01), Hayashizaki
patent: 6268133 (2001-07-01), Nisson et al.
patent: 6787305 (2004-09-01), Li et al.
patent: 44 11 588 (1995-09-01), None
patent: 44 11 594 (1995-12-01), None
patent: 0 821 058 (1998-01-01), None
patent: 0 821 059 (1998-01-01), None
patent: 0 726 310 (2003-07-01), None
patent: WO 95/20682 (1995-08-01), None
patent: WO 99/41410 (1999-08-01), None
LaVallie et al., Gene fusion expression systems inEscherichiacoil, Current Opinion in Biotechnology 1995, 6:501-506.
Aslanyan, V.M., et al., “Conformation and Thermal Stability of DNA in Aqueous Solutions of β-Alanine and γ-Aminobutyric Acid,”Biophysics 29:615-620, Pergamon Press (1984).
Buche, A., et al., “Glycine and other amino compounds prevent chromatin precipitation at physiological ionic strength,”FEBS Lett. 247:367-370, Elsevier Science B.V. (1989).
Buche, A., et al., “Organic Osmotic Effectors and Chromatin Structure,”J. Biomol. Struct. &Dynam. 8:601-618, Adenine Press (1990).
Buche, A., et al., “Effect of Organic Effectors on Chromatin Solubility, DNA-Histone H1 Interactions, DNA and Histone H1 Structures,”J. Biomol. Struct. &Dynam. 11:95-119, Adenine Press (1993).
Cánovas, D., et al., Osmoprotectants inHalomonas elongata: High-Affinity Betaine Transport System and Choline-Betaine Pathway,J. Bacteriol. 178:7221-7226, American Society for Microbiology (1996).
Cánovas, D., et al., “Isolation and Characterization of Salt-sensitive Mutants of the Moderate HalophileHalomonas elongataand Cloning of the Ectoine Synthesis Genes,”J. Biol. Chem. 272:25794-25801, American Society for Biochemistry and Molecular Biology (Oct. 1997).
Carninci, P., et al., “Thermostabilization and thermoactivation of thermolabile enzymes by trehalose and its application for the synthesis of full length cDNA,”Proc. Natl. Acad. Sci. USA 95:520-524, National Academy of Sciences (Jan. 1998).
Chambers, S. T., et al., “Dimethylthetin Can Substitute for Glycine Betaine as an Osmoprotectant Molecule forEscherichia coli,” J. Bacteriol. 169:4845-4847, American Society for Microbiology (1987).
Flock, S., et al., “Osmotic Effectors and DNA Structure: Effect of Glycine on Precipitation of DNA by Multivalent Cations.”J. Biomol. Struct. &Dynam. 13:87-102, Adenine Press (1995).
Flock, S., et al., “Dielectric Constant and Ionic Strength Effects on DNA Precipitation,”Biophys. J. 70:1456-1465, Biophysical Society (1996).
Flock, S., et al., “23Na NMR Study of the Effect of Organic Osmolytes on DNA Counterion Atmosphere,”Biophys. J. 71:1519-1529, Biophysical Society (1996).
Frackman, S., et al., “Betaine and DMSO: Enhancing Agents for PCR,”Promega Notes 65:27-29, Promega (Feb. 1998).
Gouesbet, G., et al., “Characterization of theErwinia chrysanthemiOsmoprotectant Transporter GeneousA,” J. Bacteriol. 178:447-455, American Society for Microbiology (1996).
Hengen, P.N., “Optimizing multiplex and LA-PCR with betaine,”Trends Biochem. Sci. 22:225-226, Elsevier Trends Journal (Jun. 1997).
Henke, W., et al., “Betaine improves the PCR amplification of GC-rich DNA sequences,”Nucl. Acids Res. 25:3957-3958, Oxford University Press (Oct. 1997).
Hogrefe, H., et al., “Novel PCR Enhancing Factor Improves Performance ofPfuDNA Polymerase,”Stratagene Strategies 10:93-96, Stratagene (Aug. 1997).
Houssier, C., et al., “Effects of Compensatory Solutes on DNA and Chromatin Structural Organization in Solution,”Comp. Biochem. Physiol. 117A:313-318, Elsevier Science Inc. (Jul. 1997).
Iakobashvili, R. and Lapidot, A., “Low temperature cycled PCR protocol for Klenow fragment of DNA polymerase I in the presence of proline,”Nucl. Acids Res. 27:1566-1568, Oxford University Press (Mar. 1999).
Instruction Manual for “GeneTrapper™ cDNA Positive Selection System,” Catalog No. 10356-020, GIBCO BRL, Life Technologies, Gaithersburg, MD.
Jakob, R. and Saenger, W., “Reversed-phase ion-pair chromotographic separation of ribulose 1,5-biphosphate from 3-phosphoglycerate and its application as a new enzyme assay for RuBP carboxylase/oxygenase,”FEBS Letters 183:111-114, Elsevier Science B.V. (1985).
Joung, et al., “Mutations in two Cysteine-hystidine-rich clusters in adenovirus type 2 DNA affect DNA binding,”J. Virol. 66:5788-5796, American Society for Microbiology (1992).
Kondakova, N.V., et al., “Effect of Low-molecular Amines on the Conformation and Stability of the DNA Double Helix,”Molekulyamaya Biologiya 9:742-746, Izdatelstvo Nauka, translated into English and cited inMolecular Biology, pp. 593-596, translated by the Consultants Bureau (1975).
Landre, P.A., et al., “The Use of Cosolvents to Enhance Amplification by the Polymerase Chain Reaction,” in:PCR Strategies, Innis, M.A. et al., eds., Academic Press, San Diego, pp. 3-16 (1995).
Le Rudulier, D., et al., “Molecular Biology of Osmoregulation,”Science 224:1064-1068, American Association for the Advancement of Science (1984).
Li, C.-J., et al., “Nonprotein Amino Acids from Seeds ofCycas circinalisandPhaseolus vulgaris,” Phytochem. 42:443-445, Pergamon Press (1996).
Malin, G. and A. Lapidot, “Induction of Synthesis of Tetrahydropyrimidine Derivatives inStreptomycesStrains and Their Effect onEscherichia coliin Response to Osmotic and Heat Stress,”J. Bacteriol. 178:385-395, American Society for Microbiology (1996).
Marquet, R. and C. Houssier, “Thermodynamics of Cation-Induced DNA Condensation,”J. Biomol. Struct. &Dynam. 9:159-167, Adenine Press (1991).
Mytelka, D.S. and M.J. Chamberlin, “Analysis and suppression of DNA polymerase pauses associated with a trinucleotide consensus,”Nucl. Acids Res. 24:2774-2781, Oxford University Press (1996).
Panaccio, M., et al., “FoLT PCR: A Simple PCR Protocol for Amplifying DNA Directly from Whole Blood,”BioTech. 14:238-243, Eaton Publishing Co. (1993).
Rafferty, J.A. and Fletcher, H.L., “Sequence Analysis of a Family of Highly Repeated DNA Units inStauroderus scalaris(Orthoptera),”Intl. J. Genome Res. 1:1-16, World Scientific (1992).
Rajendrakumar, C.S.V., et al., “DN
Gebeyehu Gulilat
Jessee Joel A.
Li Wu-Bo
Schuster David
Xia Jiulin
Benzion Gary
Invitrogen Corporation
Staples Mark
LandOfFree
Methods for producing polypeptides through enhanced... does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Methods for producing polypeptides through enhanced..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Methods for producing polypeptides through enhanced... will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-3918328