Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...
Patent
1997-03-17
1999-04-06
Guzo, David
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Recombinant dna technique included in method of making a...
435455, 435463, 435183, 435201, 435198, 435190, 435193, 435207, 435208, 435212, 435199, 435219, 435220, 435224, 435225, 4352523, 43525411, 43525421, 43525422, 43525423, 4352543, 4352544, 4352545, C12P 2100, C12N 119, C12N 121, C12N 910
Patent
active
058916695
ABSTRACT:
The present invention relates to methods of producing a polypeptide, comprising: (a) introducing into a respiratory-defective mutant of a cell (i) one or more first nucleic acid sequences which complement the respiratory defect and (ii) a second nucleic acid sequence which encodes the polypeptide; (b) cultivating the cell containing the first and second nucleic acid sequences in a culture medium under aerobic conditions suitable for expression of the first and second nucleic acid sequences; and (c) isolating the polypeptide from the cultivation medium of the cell. The present invention also relates to methods for disrupting a gene in a respiratory-deficient mutant cell. The present invention further relates to respiratory-deficient mutant cells and methods for obtaining such mutant cells.
REFERENCES:
patent: 4902620 (1990-02-01), Bard et al.
Schmalix et al., "Kinetics of the Intracellular Avilability of Heme after Supplementing a Heme-Deficient Yeast Mutant with 5-Aminolevulinate".
James E. Bailey, Science, vol. 252, pp. 1668-1675. Jun. 21, 1991.
Cherry Joel R.
Elrod Susan L.
Jensen Ejner Bech
Gregg Valeta A.
Guzo David
Lambiris Elias J.
Novo Nordisk A/S, Novoalle,
Novo Nordisk Biotech
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