Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...
Patent
1997-11-18
1999-09-28
Railey, II, Johnny F.
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Recombinant dna technique included in method of making a...
43525231, 435471, 435477, 435485, C12N 121, C12P 2100
Patent
active
059587288
ABSTRACT:
The present invention relates to methods of producing a polypeptide, comprising: (a) cultivating a mutant of a Bacillus cell, wherein the mutant (i) comprises a first nucleic acid sequence encoding the polypeptide and a second nucleic acid sequence comprising a modification of at least one of the genes responsible for the biosynthesis or secretion of a surfactin or isoform thereof under conditions conducive for the production of the polypeptide and (ii) the mutant produces less of the surfactin or isoform thereof than the Bacillus cell when cultured under the same conditions; and (b) isolating the polypeptide from the cultivation medium. The present invention also relates to mutants of Bacillus cells and methods for producing the mutants.
REFERENCES:
Nakano et al., Mol. Gen. Genet. 232:313-321 (1992).
D'Souza et al., J. Bact. 175(11):3502-3510 (1993).
Vollenbroich et al., J. Bact. 176(2):395-400 (1994).
Galli et al., "Characterization of the Surfactin Synthetase Multi-Enzyme Complex", Biochinica et Biophsica Acta vol. 1205 (1994) pp. 19-28.
Razafindralambo et al. "Foaming Properties of Surfactin, a Lipopeptide Biosurfactant From Bacillus Subtilis", JAOCS, vol. 73, No. 1, (1996), pp. 149-151.
Database WPI, Section Ch, Week 7417, Derwent Publications Ltd., London, GB; AN 74-31507V -- XP002058920 & JP 49001 791 A (Nat Res Inst of Brewing), Jan. 9, 1974.
Adams Lee F.
Brown Stephen
Sloma Alan
Sternberg David
Gregg Valeta A.
Lambiris Elias J
Novo NordiskBiotech, Inc.
Railey II Johnny F.
Zelson Steven T.
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