Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing compound containing saccharide radical
Patent
1998-08-19
2000-04-04
Marschel, Ardin H.
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Preparing compound containing saccharide radical
435 6, 435 912, 436501, 536 253, C12P 1734
Patent
active
060460393
ABSTRACT:
The present invention is an improved method of making a partially modified PCR product from a DNA fragment with a polymerase chain reaction (PCR). In a standard PCR process, the DNA fragment is combined with starting deoxynucleoside triphosphates, a primer, a buffer and a DNA polymerase in a PCR mixture. The PCR mixture is then reacted in the PCR producing copies of the DNA fragment. The improvement of the present invention is adding an amount of a modifier at any step prior to completion of the PCR process thereby randomly and partially modifying the copies of the DNA fragment as a partially modified PCR product. The partially modified PCR product may then be digested with an enzyme that cuts the partially modified PCR product at unmodified sites thereby producing an array of DNA restriction fragments.
REFERENCES:
patent: 5316948 (1994-05-01), Pless
patent: 5487985 (1996-01-01), McClelland et al.
patent: 5759778 (1998-06-01), Li et al.
patent: 5821356 (1998-10-01), Khan et al.
PCR with 5-methyl-dCTP replacing dCTP, KK Wong and M McClelland, Nucleic Acids Research, vol. 19, No. 5, 1991, pp. 1081-1085.
Partial Digestion with Restrictin Enzymes of Ultraviolet-Irradiated Human Genomic DNA: A Method for Identifying Restriction Site Polymorphisms, C Nobile and G Romeo, Genomics 3, 272-274 (1988).
New cloning vectors and techniques for easy and rapid restriction mappping, KD Tartof and CA Hobbs, Gene 67, 169-182 (1988).
Control of partial digestion combining the enzymes dam methylase and MboI, JD Hoheisel, D Nizetic and H Lehrach, Nucleic Acids Research, vol. 17, No. 23 (1989), pp. 9571-9582.
Determination of Fragment Order through Partial Digests and Multiple Enzyme digests, KJ Danna, Methods in Enzymology, vol. 65, pp. 449-467 (1980).
Battelle (Memorial Institute)
Marschel Ardin H.
Zimmerman Paul W.
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