Chemistry: molecular biology and microbiology – Micro-organism – per se ; compositions thereof; proces of... – Fungi
Reexamination Certificate
2005-11-10
2009-12-08
Desai, Anand U (Department: 1656)
Chemistry: molecular biology and microbiology
Micro-organism, per se ; compositions thereof; proces of...
Fungi
C530S395000
Reexamination Certificate
active
07629163
ABSTRACT:
Cell lines having genetically modified glycosylation pathways that allow them to carry out a sequence of enzymatic reactions, which mimic the processing of glycoproteins in humans, have been developed. Recombinant proteins expressed in these engineered hosts yield glycoproteins more similar, if not substantially identical, to their human counterparts. The lower eukaryotes, which ordinarily produce high-mannose containing N-glycans, including unicellular and multicellular fungi are modified to produce N-glycans such as Man5GlcNAc2or other structures along human glycosylation pathways. This is achieved using a combination of engineering and/or selection of strains which: do not express certain enzymes which create the undesirable complex structures characteristic of the fungal glycoproteins, which express exogenous enzymes selected either to have optimal activity under the conditions present in the fungi where activity is desired, or which are targeted to an organelle where optimal activity is achieved, and combinations thereof wherein the genetically engineered eukaryote expresses multiple exogenous enzymes required to produce “human-like” glycoproteins.
REFERENCES:
patent: 4414329 (1983-11-01), Wegner
patent: 4617274 (1986-10-01), Wegner
patent: 4683293 (1987-07-01), Craig
patent: 4775622 (1988-10-01), Hitzeman et al.
patent: 4808537 (1989-02-01), Stroman et al.
patent: 4812405 (1989-03-01), Lair et al.
patent: 4818700 (1989-04-01), Cregg et al.
patent: 4837148 (1989-06-01), Cregg
patent: 4855231 (1989-08-01), Stroman et al.
patent: 4857467 (1989-08-01), Sreekrishna et al.
patent: 4879231 (1989-11-01), Stroman et al.
patent: 4882279 (1989-11-01), Cregg
patent: 4885242 (1989-12-01), Cregg
patent: 4925796 (1990-05-01), Bergh et al.
patent: 4929555 (1990-05-01), Cregg et al.
patent: 4935349 (1990-06-01), McKnight et al.
patent: 5002876 (1991-03-01), Sreekrishna et al.
patent: 5004688 (1991-04-01), Craig et al.
patent: 5032516 (1991-07-01), Cregg
patent: 5032519 (1991-07-01), Paulson et al.
patent: 5047335 (1991-09-01), Paulson et al.
patent: 5122465 (1992-06-01), Cregg et al.
patent: 5135854 (1992-08-01), MacKay et al.
patent: 5166329 (1992-11-01), Cregg
patent: 5324663 (1994-06-01), Lowe
patent: 5595900 (1997-01-01), Lowe
patent: 5602003 (1997-02-01), Pierce et al.
patent: 5707828 (1998-01-01), Sreekrishna et al.
patent: 5766910 (1998-06-01), Fukuda et al.
patent: 5834251 (1998-11-01), Maras et al.
patent: 5849904 (1998-12-01), Gerardy-Schahn et al.
patent: 5854018 (1998-12-01), Hitzeman et al.
patent: 5861293 (1999-01-01), Kojiri et al.
patent: 5910570 (1999-06-01), Elhammer et al.
patent: 5945314 (1999-08-01), Prieto et al.
patent: 5945322 (1999-08-01), Gotschlich
patent: 5955347 (1999-09-01), Lowe
patent: 5955422 (1999-09-01), Lin
patent: 5962294 (1999-10-01), Paulson et al.
patent: 6017743 (2000-01-01), Tsuji et al.
patent: 6096512 (2000-08-01), Elhammer et al.
patent: 6204431 (2001-03-01), Prieto et al.
patent: 6410246 (2002-06-01), Zhu et al.
patent: 7029872 (2006-04-01), Gerngross
patent: 7064191 (2006-06-01), Shinkawa et al.
patent: 7214775 (2007-05-01), Hanai et al.
patent: 2004/0018590 (2004-01-01), Gerngross et al.
patent: 2004/0171826 (2004-09-01), Hamilton
patent: 2004/0230042 (2004-11-01), Hamilton
patent: 2005/0170452 (2005-08-01), Wildt et al.
patent: 2005/0208617 (2005-09-01), Bobrowicz et al.
patent: 2005/0260729 (2005-11-01), Hamilton
patent: 2006/0024292 (2006-02-01), Gerngross et al.
patent: 2006/0024304 (2006-02-01), Gerngross et al.
patent: 2006/0029604 (2006-02-01), Gerngross et al.
patent: 2006/0034828 (2006-02-01), Gerngross et al.
patent: 2006/0034829 (2006-02-01), Gerngross et al.
patent: 2006/0034830 (2006-02-01), Gerngross et al.
patent: 2006/0040353 (2006-02-01), Davidson et al.
patent: 2006/0078963 (2006-04-01), Gerngross
patent: 2006/0160179 (2006-07-01), Bobrowicz et al.
patent: 2006/0177898 (2006-08-01), Gerngross
patent: 2006/0211085 (2006-09-01), Bobrowicz
patent: 2006/0286637 (2006-12-01), Hamilton
patent: 2007/0105127 (2007-05-01), Gerngross
patent: 1054062 (2000-11-01), None
patent: 1211310 (2002-06-01), None
patent: 0905232 (2005-03-01), None
patent: 1522590 (2005-04-01), None
patent: 1297172 (2005-11-01), None
patent: 8-336387 (1996-12-01), None
patent: WO 96/21038 (1996-07-01), None
patent: WO 98/05768 (1998-02-01), None
patent: WO 99/31224 (1999-06-01), None
patent: WO 99/54342 (1999-10-01), None
patent: WO 01/14522 (2000-08-01), None
patent: WO 00/61739 (2000-10-01), None
patent: WO 01/25406 (2001-04-01), None
patent: WO 01/36432 (2001-05-01), None
patent: WO 01/60860 (2001-08-01), None
patent: WO 02/00856 (2002-01-01), None
patent: WO 02/097060 (2002-12-01), None
patent: WO 03/025148 (2003-03-01), None
patent: WO 03/056914 (2003-07-01), None
patent: WO 2004/074458 (2004-09-01), None
patent: WO 2004/074461 (2004-09-01), None
patent: WO 2004/074497 (2004-09-01), None
patent: WO 2004/074498 (2004-09-01), None
patent: WO 2004/074499 (2004-09-01), None
patent: WO 2007/028144 (2007-03-01), None
Biochemistry, Voet and Voet, John Wiley & Sons, 1990, pp. 266-267.
Chiba et al. (1998) The Journal of Biological Chemistry, vol. 273, p. 26298-26304.
Tatara et al. (2003) The Journal of Biological Chemistry, vol. 278, p. 25289-25294.
Fujita et al. (1997) Biochemical and Biophysical Research Communications, vol. 238, p. 779-783.
Marriam-Webster, definition of domain.
Lee et al. (1997) Biotechnol. Prog., vol. 13, p. 368-373.
Berninsone et al. (1994) The Journal of Biological Chemistry, vol. 269, p. 207-211.
Puglielli et al., J. Biol. Chem., vol. 274 (1999), pp. 35596-35600, “Reconstitution, identification, and purification of the rat liver Golgi membrane . . . ”.
Guillen et al., PNAS, vol. 95 (1998), pp. 7888-7892, “Mammalian Golgi apparatus UDP-N-acetylglucosamine transporter: . . . ”.
Chiba et al., J. Biol. Chem., vol. 273 (1998), pp. 26298-26304, “Production of human compatible high mannose-type (Man5GlcNAc2) sugar chains inSaccharomyces cerevisiae”.
Berninsone et al., J. Biol. Chem., vol. 272 (1997), pp. 12616-12619, “Functional expression of the murine Golgi CMP-sialic acid transporter inSaccharomyces cerevisiae”.
Berninsone et al., J. Biol. Chem., vol. 270 (1995), pp. 14564-14567, “Regulation of yeast Golgi glycosylation”.
Nakayama et al., The EMBO Journal, vol. 11 (1992), pp. 2511-2519, “OCH1 encodes a novel membrane bound mannosyltransferase: . . . ”.
Nikawa et al., Gene, vol. 171 (1996), pp. 107-111, “Structural and functional conservation of human and yeast HCP1 genes which can suppres the growth defect . . . ”.
Kalsner et al., Glycoconjugate Journal, vol. 12 (1995), pp. 360-370, “Insertion intoAspergilus nidulansof functional UDP-GlcNAc: alpha3-D-mannoside beta-1,2-N-acetylglucosaminyl-transferase 1 . . . ”.
Martinet et al., Biotechnol. Letters, vol. 20 (1998), pp. 1171-1177, “Modification of the protein glycosylation pathway . . . ”.
Kainuma et al., Glycobiology, vol. 9 (1999), pp. 133-141, “Coexpression of alpha1,2 galactosyltransferase and UDP-galactose transporter . . . ”.
Jarvis et al., Curr. Opin. in Biotech., vol. 9 (1998), pp. 528-533, “Engineering N-glycosylation pathways in the baculovirus-insert cell system”.
Ishida et al., J. Biochem., vol. 126 (1999), pp. 68-77, “Molecular cloning and functional expression of the human Golgi . . . ”.
Ichishima et al., Biochem. Journal, vol. 339 (1999), pp. 589-597, “Molecular and enzymic properties of recombinant 1,2-alpha-mannosidase . . . ”.
Harkki et al., Bio-Tech., vol. 7 (1989), pp. 596-603, “A novel fungal expression system: Secretion of active calf chymosin . . . ”.
Eckhardt et al., Eur. J. Biochem., vol. 248 (1997), pp. 187-192, “Molecular cloning of the hamster CMP-sialic acid transporter”.
Graham et al., J. Cell Biol., vol. 114 (1991), pp. 207-218, “Compartmental organization of Golgi-specific protein modification . . . ”.
Eades et al., Gene, vo
Desai Anand U
Fitch Catherine D.
GlycoFi, Inc.
Reilly John David
LandOfFree
Methods for producing modified glycoproteins does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Methods for producing modified glycoproteins, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Methods for producing modified glycoproteins will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-4091020