Methods for producing modified glycoproteins

Details Methods for producing modified glycoproteins Methods for producing modified glycoproteins

2011-04-12

2011-04-12

Desai, Anand U (Department: 1656)

Drug, bio-affecting and body treating compositions

Designated organic active ingredient containing

Peptide containing doai

C514S042000, C530S395000, C530S350000

Reexamination Certificate

active

07923430

ABSTRACT:
Cell lines having genetically modified glycosylation pathways that allow them to carry out a sequence of enzymatic reactions, which mimic the processing of glycoproteins in humans, have been developed. The lower eukaryotes, which ordinarily produce high-mannose containing N-glycans, including unicellular and multicellular fungi are modified to produce N-glycans such as Man5GlcNAc2or other structures along human glycosylation pathways. This is achieved using a combination of engineering and/or selection of strains which: do not express certain enzymes which create the undesirable complex structures characteristic of the fungal glycoproteins, which express exogenous enzymes selected either to have optimal activity under the conditions present in the fungi where activity is desired, or which are targeted to an organelle where optimal activity is achieved, and combinations thereof wherein the genetically engineered eukaryote expresses multiple exogenous enzymes required to produce “human-like” glycoproteins.

REFERENCES:
patent: 4414329 (1983-11-01), Wegner
patent: 4617274 (1986-10-01), Wegner
patent: 4683293 (1987-07-01), Craig
patent: 4775622 (1988-10-01), Hitzeman et al.
patent: 4808537 (1989-02-01), Stroman et al.
patent: 4812405 (1989-03-01), Lair et al.
patent: 4818700 (1989-04-01), Cregg et al.
patent: 4837148 (1989-06-01), Cregg
patent: 4855231 (1989-08-01), Stroman et al.
patent: 4857467 (1989-08-01), Sreekrishna et al.
patent: 4879231 (1989-11-01), Stroman et al.
patent: 4882279 (1989-11-01), Cregg
patent: 4885242 (1989-12-01), Cregg
patent: 4925796 (1990-05-01), Bergh et al.
patent: 4929555 (1990-05-01), Cregg et al.
patent: 4935349 (1990-06-01), McKnight et al.
patent: 5002876 (1991-03-01), Sreekrishna et al.
patent: 5004688 (1991-04-01), Craig et al.
patent: 5032516 (1991-07-01), Cregg
patent: 5032519 (1991-07-01), Paulson et al.
patent: 5047335 (1991-09-01), Paulson et al.
patent: 5122465 (1992-06-01), Cregg et al.
patent: 5135854 (1992-08-01), MacKay et al.
patent: 5166329 (1992-11-01), Cregg
patent: 5272066 (1993-12-01), Bergh et al.
patent: 5324663 (1994-06-01), Lowe
patent: 5595900 (1997-01-01), Lowe
patent: 5602003 (1997-02-01), Pierse et al.
patent: 5683899 (1997-11-01), Stuart
patent: 5707828 (1998-01-01), Sreekrishna et al.
patent: 5766910 (1998-06-01), Fukuda et al.
patent: 5834251 (1998-11-01), Maras et al.
patent: 5844093 (1998-12-01), Kettleborough et al.
patent: 5849904 (1998-12-01), Gerardy-Schahn et al.
patent: 5854018 (1998-12-01), Hitzemane et al.
patent: 5861293 (1999-01-01), Kojiri et al.
patent: 5910570 (1999-06-01), Elhammer et al.
patent: 5945314 (1999-08-01), Prieto et al.
patent: 5945322 (1999-08-01), Gotschlich
patent: 5955347 (1999-09-01), Lowe
patent: 5955422 (1999-09-01), Lin
patent: 5962294 (1999-10-01), Paulson et al.
patent: 6017743 (2000-01-01), Tsuji et al.
patent: 6069235 (2000-05-01), Davis et al.
patent: 6096512 (2000-08-01), Elhammer et al.
patent: 6204431 (2001-03-01), Prieto et al.
patent: 6300113 (2001-10-01), Landry
patent: 6410246 (2002-06-01), Zhu et al.
patent: 6602684 (2003-08-01), Umana et al.
patent: 6946292 (2005-09-01), Kanda et al.
patent: 7029872 (2006-04-01), Gerngross
patent: 7064191 (2006-06-01), Shinkawa et al.
patent: 7214775 (2007-05-01), Hanai et al.
patent: 7326681 (2008-02-01), Gerngross
patent: 7332299 (2008-02-01), Hamilton
patent: 7365163 (2008-04-01), Hanna
patent: 7449308 (2008-11-01), Gerngross
patent: 2002/0137134 (2002-09-01), Gerngross
patent: 2003/0157108 (2003-08-01), Presta
patent: 2003/0175884 (2003-09-01), Umana et al.
patent: 2004/0018590 (2004-01-01), Gerngross et al.
patent: 2004/0171826 (2004-09-01), Hamilton
patent: 2004/0191256 (2004-09-01), Raju
patent: 2004/0229306 (2004-11-01), Nett
patent: 2004/0230042 (2004-11-01), Hamilton
patent: 2005/0079605 (2005-04-01), Umana et al.
patent: 2005/0170452 (2005-08-01), Wildt et al.
patent: 2005/0208617 (2005-09-01), Bobrowicz et al.
patent: 2005/0260729 (2005-11-01), Hamilton
patent: 2005/0265988 (2005-12-01), Choi et al.
patent: 2006/0024292 (2006-02-01), Gerngross et al.
patent: 2006/0024304 (2006-02-01), Gerngross et al.
patent: 2006/0029604 (2006-02-01), Gerngross et al.
patent: 2006/0034828 (2006-02-01), Gerngross et al.
patent: 2006/0034829 (2006-02-01), Gerngross et al.
patent: 2006/0034830 (2006-02-01), Gerngross et al.
patent: 2006/0040353 (2006-02-01), Davidson et al.
patent: 2006/0148035 (2006-07-01), Gerngross
patent: 2006/0160179 (2006-07-01), Bobrowicz et al.
patent: 2006/0177898 (2006-08-01), Gerngross
patent: 2006/0211085 (2006-09-01), Bobrowicz
patent: 2006/0257399 (2006-11-01), Gerngross et al.
patent: 2006/0286637 (2006-12-01), Hamilton
patent: 2007/0037248 (2007-02-01), Bobrowicz et al.
patent: 2007/0105127 (2007-05-01), Gerngross
patent: 2007/0154591 (2007-07-01), Andersen
patent: 2008/0274162 (2008-11-01), Nessa et al.
patent: 0 905 232 (1999-03-01), None
patent: 1 054 062 (2000-11-01), None
patent: 1176195 (2002-01-01), None
patent: 1 211 310 (2002-06-01), None
patent: 1 239 047 (2002-09-01), None
patent: 1297172 (2003-04-01), None
patent: 1522590 (2005-04-01), None
patent: 8-336387 (1996-12-01), None
patent: 11-103158 (1999-04-01), None
patent: WO 96/21038 (1996-07-01), None
patent: WO 98/05768 (1998-02-01), None
patent: WO 99/31224 (1999-06-01), None
patent: WO 99/40208 (1999-08-01), None
patent: WO 99/54342 (1999-10-01), None
patent: WO 00/61739 (2000-10-01), None
patent: WO 01/14522 (2001-03-01), None
patent: WO 01/25406 (2001-04-01), None
patent: WO 01/36432 (2001-05-01), None
patent: WO 01/60860 (2001-08-01), None
patent: WO 02/00856 (2002-01-01), None
patent: WO 02/00879 (2002-01-01), None
patent: WO 02/097060 (2002-12-01), None
patent: WO 03/011878 (2003-02-01), None
patent: WO 03/025148 (2003-03-01), None
patent: WO 03/031464 (2003-04-01), None
patent: WO 03/056914 (2003-07-01), None
patent: WO 2004/003194 (2004-01-01), None
patent: WO 2004/074458 (2004-09-01), None
patent: WO 2004/074461 (2004-09-01), None
patent: WO 2004/074497 (2004-09-01), None
patent: WO 2004/074498 (2004-09-01), None
patent: WO 2004/074499 (2004-09-01), None
patent: WO 2004/104165 (2004-12-01), None
patent: WO 2005/065019 (2005-07-01), None
patent: WO 2005/090552 (2005-09-01), None
patent: WO 2005/100584 (2005-10-01), None
patent: WO 2005/106010 (2005-11-01), None
patent: WO 2006/014679 (2006-02-01), None
patent: WO 2006/014683 (2006-02-01), None
patent: WO 2006/014685 (2006-02-01), None
patent: WO 2006/014725 (2006-02-01), None
patent: WO 2006/014726 (2006-02-01), None
patent: WO 2006/071280 (2006-07-01), None
patent: WO 2006/071856 (2006-07-01), None
patent: WO 2007/028144 (2007-03-01), None
patent: WO 2007/029054 (2007-03-01), None
Pakula et al. (2000) Monitoring the kinetics of glycoprotein synthesis and secretion in the filamentous fungusTrichoderma reesei: cellobiohydrolase I(CBHI) as a model protein, Microbiology, vol. 146, Part 1, pp. 223-232.
Lowder et al. (1985) Monoclonal antibodies—therapeutic and diagnostic uses in malignancy, West J. Med., vol. 143, No. 6, pp. 810-818.
Hard et al. (1990) Isolation and structure determination of the intact sialylated N-linked carbohydrate chains of recombinant human follitropin expressed in Chinese hamster ovary cells, Eur. J. Biochem., vol. 193, No. 1, pp. 263-271.
Montesino et al. (1999) Characterization of the oligosaccharides assembled on thePichia pastoris-expressed recombinant aspartic protease, Glycobiology, vol. 9, No. 10, pp. 1037-1043.
Chandrasekaran et al. (1984) Purification and properties of alpha-D-mannose:beta-1,2-N-acetylglucosaminyl-transferases and alpha-D-mannosidases from human adenocarcinoma, Cancer Res., vol. 44, No. 9, pp. 4059-4068.
Juranic et al. (2005) Antiproliferative action of water extracts of seeds of pulp of five different raspberry cultivars, Food Chem., vol. 93,pp. 39-45.
Gerngross T. U. (2004) Advances in the production of human therapeutic proteins in yeasts and filamentous fungi, Nat. Biotechnol., vol. 22, No. 11, pp. 1409-1414.
Reichner et al. (1998) Recycling cell surface glycoproteins undergo limited oligosaccharide reprocessing in LEC1

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