Methods for producing modified glycoproteins

Details Methods for producing modified glycoproteins Methods for producing modified glycoproteins

2011-07-19

2011-07-19

Desai, Anand U (Department: 1656)

Chemistry: molecular biology and microbiology

Micro-organism, per se ; compositions thereof; proces of...

Fungi

C530S395000

Reexamination Certificate

active

07981660

ABSTRACT:
Cell lines having genetically modified glycosylation pathways that allow them to carry out a sequence of enzymatic reactions, which mimic the processing of glycoproteins in humans, have been developed. Recombinant proteins expressed in these engineered hosts yield glycoproteins more similar, if not substantially identical, to their human counterparts. The lower eukaryotes, which ordinarily produce high-mannose containing N-glycans, including unicellular and multicellular fungi are modified to produce N-glycans such as Man5GlcNAc2or other structures along human glycosylation pathways. This is achieved using a combination of engineering and/or selection of strains which: do not express certain enzymes which create the undesirable complex structures characteristic of the fungal glycoproteins, which express exogenous enzymes selected either to have optimal activity under the conditions present in the fungi where activity is desired, or which are targeted to an organelle where optimal activity is achieved, and combinations thereof wherein the genetically engineered eukaryote expresses multiple exogenous enzymes required to produce “human-like” glycoproteins.

REFERENCES:
patent: 4414329 (1983-11-01), Wegner
patent: 4617274 (1986-10-01), Wegner
patent: 4683293 (1987-07-01), Craig
patent: 4775622 (1988-10-01), Hitzeman et al.
patent: 4808537 (1989-02-01), Stroman et al.
patent: 4812405 (1989-03-01), Lair et al.
patent: 4818700 (1989-04-01), Cregg et al.
patent: 4837148 (1989-06-01), Cregg
patent: 4855231 (1989-08-01), Stroman et al.
patent: 4857467 (1989-08-01), Sreekrishna et al.
patent: 4879231 (1989-11-01), Stroman et al.
patent: 4882279 (1989-11-01), Cregg
patent: 4885242 (1989-12-01), Cregg
patent: 4925796 (1990-05-01), Bergh et al.
patent: 4929555 (1990-05-01), Cregg et al.
patent: 4935349 (1990-06-01), McKnight et al.
patent: 5002876 (1991-03-01), Sreekrishna et al.
patent: 5004688 (1991-04-01), Craig et al.
patent: 5032516 (1991-07-01), Cregg
patent: 5032519 (1991-07-01), Paulson et al.
patent: 5047335 (1991-09-01), Paulson et al.
patent: 5122465 (1992-06-01), Cregg et al.
patent: 5135854 (1992-08-01), MacKay et al.
patent: 5166329 (1992-11-01), Cregg
patent: 5272066 (1993-12-01), Bergh et al.
patent: 5324663 (1994-06-01), Lowe
patent: 5595900 (1997-01-01), Lowe
patent: 5602003 (1997-02-01), Pierce et al.
patent: 5683899 (1997-11-01), Stuart
patent: 5707828 (1998-01-01), Sreekrishna et al.
patent: 5766910 (1998-06-01), Fukuda et al.
patent: 5834251 (1998-11-01), Maras et al.
patent: 5844093 (1998-12-01), Kettleborough et al.
patent: 5849904 (1998-12-01), Gerardy-Schahn et al.
patent: 5854018 (1998-12-01), Hitzemane et al.
patent: 5861293 (1999-01-01), Kojiri et al.
patent: 5910570 (1999-06-01), Elhammer et al.
patent: 5945314 (1999-08-01), Prieto et al.
patent: 5945322 (1999-08-01), Gotschlich
patent: 5955347 (1999-09-01), Lowe
patent: 5955422 (1999-09-01), Lin
patent: 5962294 (1999-10-01), Paulson et al.
patent: 6017743 (2000-01-01), Tsuji et al.
patent: 6069235 (2000-05-01), Davis et al.
patent: 6096512 (2000-08-01), Elhammer et al.
patent: 6204431 (2001-03-01), Prieto et al.
patent: 6300113 (2001-10-01), Landry
patent: 6410246 (2002-06-01), Zhu et al.
patent: 6602684 (2003-08-01), Umana et al.
patent: 6946292 (2005-09-01), Kanda et al.
patent: 7029872 (2006-04-01), Gerngross
patent: 7064191 (2006-06-01), Shinkawa et al.
patent: 7214775 (2007-05-01), Hanai et al.
patent: 7259007 (2007-08-01), Bobrowicz
patent: 7326681 (2008-02-01), Gerngross
patent: 7332299 (2008-02-01), Hamilton
patent: 7365163 (2008-04-01), Hanna
patent: 7449308 (2008-11-01), Gerngross
patent: 7465577 (2008-12-01), Bobrowicz
patent: 7514253 (2009-04-01), Nett
patent: 7517670 (2009-04-01), Umana
patent: 7598055 (2009-10-01), Bobrowicz et al.
patent: 7625756 (2009-12-01), Hamilton
patent: 7629163 (2009-12-01), Gerngross
patent: 2003/0157108 (2003-08-01), Presta
patent: 2003/0175884 (2003-09-01), Umana et al.
patent: 2004/0191256 (2004-09-01), Raju
patent: 2005/0170452 (2005-08-01), Wildt et al.
patent: 2005/0260729 (2005-11-01), Hamilton
patent: 2005/0265988 (2005-12-01), Choi et al.
patent: 2006/0024292 (2006-02-01), Gerngross et al.
patent: 2006/0024304 (2006-02-01), Gerngross et al.
patent: 2006/0029604 (2006-02-01), Gergross et al.
patent: 2006/0034828 (2006-02-01), Gerngross et al.
patent: 2006/0034829 (2006-02-01), Gerngross et al.
patent: 2006/0034830 (2006-02-01), Gerngross et al.
patent: 2006/0040353 (2006-02-01), Davidson et al.
patent: 2006/0177898 (2006-08-01), Gerngross
patent: 2006/0257399 (2006-11-01), Gerngross et al.
patent: 2006/0286637 (2006-12-01), Hamilton
patent: 2007/0037248 (2007-02-01), Bobrowicz et al.
patent: 2007/0105127 (2007-05-01), Gerngross
patent: 2007/0154591 (2007-07-01), Andersen
patent: 2008/0274162 (2008-11-01), Nessa et al.
patent: 0 905 232 (1999-03-01), None
patent: 1 054 062 (2000-11-01), None
patent: 1176195 (2002-01-01), None
patent: 1 211 310 (2002-06-01), None
patent: 1 239 047 (2002-09-01), None
patent: 1297172 (2003-04-01), None
patent: 1522590 (2005-04-01), None
patent: 8-336387 (1996-12-01), None
patent: 11-103158 (1999-04-01), None
patent: WO 96/21038 (1996-07-01), None
patent: WO 98/05768 (1998-02-01), None
patent: WO 99/31224 (1999-06-01), None
patent: WO 99/40208 (1999-08-01), None
patent: WO 99/54342 (1999-10-01), None
patent: WO 00/61739 (2000-10-01), None
patent: WO 01/14522 (2001-03-01), None
patent: WO 01/25406 (2001-04-01), None
patent: WO 01/36432 (2001-05-01), None
patent: WO 01/60860 (2001-08-01), None
patent: WO 02/00856 (2002-01-01), None
patent: WO 02/00879 (2002-01-01), None
patent: WO 02/97060 (2002-12-01), None
patent: WO 03/011878 (2003-02-01), None
patent: WO 03/025148 (2003-03-01), None
patent: WO 03/031464 (2003-04-01), None
patent: WO 03/056914 (2003-07-01), None
patent: WO 2004/003194 (2004-01-01), None
patent: WO 2004/074458 (2004-09-01), None
patent: WO 2004/074461 (2004-09-01), None
patent: WO 2004/074497 (2004-09-01), None
patent: WO 2004/074498 (2004-09-01), None
patent: WO 2004/074499 (2004-09-01), None
patent: WO 2004/104165 (2004-12-01), None
patent: WO 2005/065019 (2005-07-01), None
patent: WO 2005/090552 (2005-09-01), None
patent: WO 2005/100584 (2005-10-01), None
patent: WO 2005/106010 (2005-11-01), None
patent: WO 2006/014679 (2006-02-01), None
patent: WO 2006/014683 (2006-02-01), None
patent: WO 2006/014685 (2006-02-01), None
patent: WO 2006/014725 (2006-02-01), None
patent: WO 2006/071280 (2006-07-01), None
patent: WO 2006/071856 (2006-07-01), None
patent: WO 2006/014726 (2006-09-01), None
patent: WO 2007/028144 (2007-03-01), None
patent: WO 2007/029054 (2007-03-01), None
Chiba et al., “Production of Human Compatible High Mannose-type (Man5GlcAc2) Sugar Chains inSaccharomyces cerevisiae”, The Journal of Biological Chemistry 273(41): 26298-26304 (Oct. 1998).
Abeijon et al., “Molecular Cloning of the Golgi apparatus uridine diphosphate-N-acetylglucosamine transporter fromKluyveromyces lactis,” Proc. Natl. Acad. Sci. USA93:5963-5968 (1996).
Adachi et al., “Mus Musculus Adult Male Testis cDNA, Riken full length enriched library, clone: 4931438M07 product: mannosidase 2, alpha 2, full insert sequence” XP002293645, Database accession No. AK029913 Abstract, Database EMBL, Dec. 21, 2002.
Alani et al., “A Method for Gene Disruption that Allows Repeated Use of URA3 Selection in the Construction of Multiply Disrupted Yeast Strains,” Genetics 116, 541-545, Aug. 1987.
Abdel-Salam et al., “Expression of mouse anticreatine kinase (MAK33) monoclonal antibody in the yeast Hansenula Polymorpha”, App. Microbiol. Biotechnol. 56:157-164 (2001).
Allison, Daniel S., et al., “Mutations in the Signal Sequence of Prepro-α-Factor Inhibit Both Translocation into the Endoplasmic Reticulum and Processing by Signal Peptide in Yeast Cells,” Molecular and Cellular Biology, vol. 9(11):4977-4985 (1989).
Altman et al., “Processing of Asparagine-linked Oligosaccharides in Insect Cells: Evidence for Alpha-Mannosidase II,”Glycoconj. J12(2):15

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