Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues – Blood proteins or globulins – e.g. – proteoglycans – platelet...
Patent
1998-03-30
2000-10-31
Ketter, James
Chemistry: natural resins or derivatives; peptides or proteins;
Proteins, i.e., more than 100 amino acid residues
Blood proteins or globulins, e.g., proteoglycans, platelet...
C12P 2108
Patent
active
061404713
ABSTRACT:
Methods, recombinant host cells and kits are disclosed for the production of members of specific binding pairs (sbp), e.g. antibodies, using display on the surface of secreted replicable genetic display packages (rgdps), e.g. filamentous phage. To produce a library of great diversity, recombination occurs between first and second vectors comprising nucleic acid encoding first and second polypeptide chains of sbp members respectively, thereby producing recombinant vectors each encoding both a first and a second polypeptide chain component of an sbp member. The recombination may take place in vitro or intracellularly and may be site-specific, e.g. involving use of the loxP sequence and mutants thereof. Recombination may take place after prior screening or selecting for rgdps displaying sbp members which bind complementary sbp member of interest.
REFERENCES:
patent: 4190495 (1980-02-01), Curtiss, III
patent: 4816397 (1989-03-01), Bass et al.
patent: 4946778 (1990-08-01), Ladner et al.
patent: 5223409 (1993-06-01), Ladner et al.
patent: 5733743 (1998-03-01), Johnson et al.
Abremski, K. et al., "Studies on the Properties of P1 Site-Specific Recombination: Evidence for Topologically Unlinked Products following Recombination," Cell, 32:1301-1311 (Apr. 1983).
Armstrong, J. et al., "Domain Structure of Bacteriophage fd Adsorption Protein," FEBS Letters, 135(1):167-172 (1981).
Bass, S. et al., "Hormone Phage: An Enrichment Method for Variant Proteins with Altered Binding Properties," Proteins, 8(4):309-314 (1990).
Better, M. et al., "Escherichia coli Secretion of an Active Chimeric Antibody Fragment," Science, 240:1041-1043 (1988).
Bird, R. et al., "Single-Chain Antigen-Binding Proteins," Science, 242:423-426 (1988).
Boyd, A.C., "Turbo Cloning: A Fast, Efficient Method for Cloning PCR Products and Other Blunt-ended DNA Fragments into Plasmids," Nucleic Acids Research, 21(4):817-821 (1993).
Carter, P. et al., "Improved oligonucleotide site-directed mutagenesis using M13 vectors," Nucleic Acids Research, 13(12):4431-4443 (1985).
Casali, P. et al., "Human Monoclonals from Antigen-Specific Selection of B Lymphocytes and Transformation by EBV," Science, 234:476-479 (1986).
Cathala, G. et al., "Laboratory Methods A Method for Isolation of Intact, Translationally Active Ribonucleic Acid," DNA, 2(4):329-335 (1983).
Chiang, Y.L. et al., "Direct cDNA Cloning of the Rearranged Immunoglobulin Varialble Region," BioTechniques, 7(4):360-366 (1989).
Clackson, T. et al., "Making antibody fragments using phage display libraries," Nature, 352:624-628 (1991).
Clonetech, cDNA & Genomic Libraries, Clonetech Laboratories, Inc., Palo Alto, California, USA, pp. 75-96 and 192 (1995/1996).
Cregg, J.M. et al., "Short Communication: Use of Site-Specific Recombination to Regenerate Selectable Markers," Molecular Gen. Genet., 219:320-323 (1989).
Dale, E.C. et al., "Gene Transfer with Subsequent Removal of the Selection Gene from the Host Genome," Proc. Natl. Acad. Sci., USA, 88:10558-10562 (Dec. 1991).
De la Cruz, V.F. et al., "Immunogenicity and Epitope Mapping of Foreign Sequences via Genetically Engineered Filamentous Phage," J. Biological Chemistry, 263(9):4318-4322 (Mar. 25, 1988).
Dower, W.J. et al., "High Efficiency Transformation of E. coli by High Voltage Electroporation," Nucleic Acids Research, 16(13):6127-6145 (1988).
Fuchs, P. et al., "Targeting Recombinant Antibodies to the Surface of Escherichia coli: Fusion to a Peptidoglycan Associated Lipoprotein," BioTechnology, 9:1369-1373 (1991).
Fukushige, S. et al., "Genomic Targeting with a Positive-selection lox Integration Vector Allows Highly Reproducible Gene Expression in Mammalian Cells," Proc. Natl. Acad. Sci., 89:7905-7909 (1992).
Gage et al., "A Cell-Free Recombination System for Site-Specific Integration of Multigenic Shuttle Plasmids into the Herpes Simplex Type 1 Genome," J. Virology, 66(9):5509-5515 (Sep., 1992).
Gherardi, E. et al., "A single-step procedure for cloning and selection of antibody-secreting hybridomas," J. Immunological Methods, 126:61-68 (1990).
Grant, R.A. et al., "Structure of the Filamentous Bacteriophage fl," J. Biological Chemistry, 256(1):539-546 (1981).
Griffiths, A.D. et al., "Isolation of High Affinity Human Antibodies Directly from Large Synthetic Repertoires," EMBO J., 13(14):3245-3260 (1994).
Griffths, G.M. et al., "Somatic mutation and the maturation of immune response to 2-phenyl oxazolone," Nature, 312:271-275 (1984).
Harlow, E. et al., in Antibodies A Laboratory Manual, Cold Spring Harbor Laboratory, Cold Spring Harbor, New York, pp. 174-195, 216 (1988).
Hoekstra et al., "A Tn3 Derivative That Can Be Used to Make Short In-Frame Insertions Within Genes," Proc. Natl. Acad. Sci., USA, 88:5457-5461 (Jun., 1991).
Hoess, R.H. et al., "The Cre-lox Recombination System," in Nucleic Acids and Molecular Biology, Eckstein et al., (eds.), vol. 4, Springer-Verlag, Berlin, Heidelberg, pp. 99-109 (1990).
Hoess, R.H. et al., "The Role of the loxP Spacer Region in P1 Site-specific Recombination," Nucleic Acids Research, 14(5):2287-2300 (1986).
Hoogenboom, H.R. et al., "Multi-subunit proteins on the surface of filamentous phage: methodologies for displaying antibody (Fab) heavy and light chains," Nucleic Acids Research, 19(15):4133-4137 (1991).
Huse, W.D. et al., "Generation of a Large Combinatorial Library of the Immunoglobulin Repertoire in Phage Lambda," Science, 246:1257-1281 (1989).
Huston, J.S. et al., "Protein engineering of antibody binding sites: Recovery of specific activity in an anti-digoxin single-chain Fv analogue produced in Escherichia coli," Proc. Natl. Acad. Sci., USA, 85:5879-5833 (1988).
Johnston, S, et al., "Interference Between M13 and oriM13 Plasmids is Mediated by a Replication Enhancer Sequence Near the Viral Strand Origin," J. Molecular Biology, 177:685-700 (1984).
Kang, A.S. et al., "Linkage of Recognition and Replication Functions by Assembly Combinatorial Antibody Fab Libraries Along Phage Surface," Proc. Natl. Acad. Sci., USA, 88(10):4363-4366 (May 15, 1991).
Klauser, T. et al., "Extracellular transport of cholera toxin B subunit using Neisseria IgA protease .beta.-domain: conformation-dependent outer membrane translocation," EMBO, 9(6):1991-1999 (1990).
Kornberg, A., in DNA Replication, W.H. Freeman and Co., San Francisco, pp. 479-496 (1980).
Landy, A., "Mechanistic and Structural Complexity in the Site-specific Recombination Pathways of Int and FLP," Current Opinion in Genetics and Development, 3:699-707 (1993).
Lane, D.P. et al., "Epitope Mapping Using Bacteriophage Peptide Libraries," Current Opin. Immunol., 5:268-271 (1993).
Larrick, J.W. et al., "Rapid Cloning of Rearranged Immunoglobulin Genes from Human Hybridoma CELSS Using Mixed Primers and the Polymerase Chain Reaction," Biochemical and Biophysical Research Communications, 160(3):1250-1256 (1989).
Lesk, A. et al., "Elbow motion in the immunoglobulins involves a molecular ball-and-socket joint," Nature, 335:188-190 (1988).
Marks, J.D. et al., "By-passing Immunization Human Antibodies from V-gene Libraries Displayed on Phage," J. Molecular Biology, 222:581-597 (1991).
Maruyama et al., "A Selective .lambda. Phage Cloning Vector With Automatic Excision of the Insert in a Plasmid," Gene, 120:135-141 (1992).
McCafferty, J. et al., "Phage Antibodies: Filamentous Phage Displaying Antibody Variable Domains," Nature, 348:552-554 (1990).
Milstein, C., "The Croonian Lecture, 1989. Antibodies: A Paradigm for the Biology of Molecular Recognition," Proc. R. Soc. London, B. Biol. Sci., 239:1-16 (1990).
Neidhardt, F.C. (Ed.), in Escherichia coli and Salmonella typhimurium, Cellular and Molecular Biology, American Society of Microbiology, Washington, D.C., pp. 1034-1043, 1054-1060, 1061-1070 (1987).
Ohlson, S. et al., "Novel Approach to Affinity Chromatography Using "Weak" Monoclonal Antibodies," Analytical Biochemistry, 169:204-208 (1988).
Orban, P.C. et al., "Tissue- and Site-specific DNA Recombination in Transgenic Mice," Proc. Natl. Acad. Sci., USA, 89:6861-6865 (Aug. 1992).
Orlandi, R. et al., "Cloning immunoglobulin variable domains for expression by th
Griffiths Andrew David
Johnson Kevin Stuart
Smith Andrew John Hammond
Waterhouse Peter Michael
Winter Gregory Paul
Cambridge Antibody Technology Ltd.
Ketter James
Medical Research Council
LandOfFree
Methods for producing members of specific binding pairs does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Methods for producing members of specific binding pairs, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Methods for producing members of specific binding pairs will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-2053354