Methods for producing members of specific binding pairs

Details Methods for producing members of specific binding pairs Methods for producing members of specific binding pairs

1999-10-13

2009-12-22

Steele, Amber D. (Department: 1639)

Combinatorial chemistry technology: method, library, apparatus

Method of screening a library

By measuring the ability to specifically bind a target...

C435S005000, C435S006120, C435S007100

Reexamination Certificate

active

07635666

ABSTRACT:
A member of a specific binding pair (sbp) is identified by expressing DNA encoding a genetically diverse population of such sbp members in recombinant host cells in which the sbp members are displayed in functional form at the surface of a secreted recombinant genetic display package (rgdp) containing DNA encoding the sbp member or a polypeptide component thereof, by virtue of the sbp member or a polypeptide component thereof being expressed as a fusion with a capsid component of the rgdp. The displayed sbps may be selected by affinity with a complementary sbp member, and the DNA recovered from selected rgdps for expression of the selected sbp members. Antibody sbp members may be thus obtained, with the different chains thereof expressed, one fused to the capsid component and the other in free form for association with the fusion partner polypeptide. A phagemid may be used as an expression vector, with said capsid fusion helping to package the phagemid DNA. Using this method libraries of DNA encoding respective chains of such multimeric sbp members may be combined, thereby obtaining a much greater genetic diversity in the sbp members than could easily be obtained by conventional methods.

REFERENCES:
patent: 4593002 (1986-06-01), Dulbecco
patent: 4704692 (1987-11-01), Ladner
patent: 4816397 (1989-03-01), Boss et al.
patent: 4946778 (1990-08-01), Ladner et al.
patent: 5091513 (1992-02-01), Huston et al.
patent: 5223409 (1993-06-01), Ladner et al.
patent: 5258289 (1993-11-01), Davis et al.
patent: 5260203 (1993-11-01), Ladner et al.
patent: 5403484 (1995-04-01), Ladner et al.
patent: 5427908 (1995-06-01), Dower et al.
patent: 5534617 (1996-07-01), Cunningham et al.
patent: 5567610 (1996-10-01), Borrebaeck et al.
patent: 5580717 (1996-12-01), Dower et al.
patent: 5658727 (1997-08-01), Barbas et al.
patent: 5688666 (1997-11-01), Bass et al.
patent: 5698426 (1997-12-01), Huse
patent: 5750373 (1998-05-01), Garrard et al.
patent: 5759817 (1998-06-01), Barbas
patent: 5770356 (1998-06-01), Light et al.
patent: 5780225 (1998-07-01), Wigler et al.
patent: 5780279 (1998-07-01), Matthews et al.
patent: 5814476 (1998-09-01), Kauffman et al.
patent: 5821047 (1998-10-01), Garrard et al.
patent: 5837242 (1998-11-01), Holliger et al.
patent: 5837500 (1998-11-01), Ladner et al.
patent: 5840479 (1998-11-01), Little et al.
patent: 5846765 (1998-12-01), Matthews et al.
patent: 5849500 (1998-12-01), Breitling et al.
patent: 5855885 (1999-01-01), Smith et al.
patent: 5858657 (1999-01-01), Winter et al.
patent: 5871907 (1999-02-01), Winter et al.
patent: 5885793 (1999-03-01), Griffiths et al.
patent: 5935823 (1999-08-01), Fowlkes et al.
patent: 5948635 (1999-09-01), Kay et al.
patent: 5955358 (1999-09-01), Huse
patent: 5969108 (1999-10-01), McCafferty et al.
patent: 5985588 (1999-11-01), Breitling et al.
patent: 6172197 (2001-01-01), McCafferty et al.
patent: 6225447 (2001-05-01), Winter et al.
patent: 6291650 (2001-09-01), Winter et al.
patent: 6331415 (2001-12-01), Cabilly et al.
patent: 6521404 (2003-02-01), Griffiths et al.
patent: 6544731 (2003-04-01), Griffiths et al.
patent: 6555313 (2003-04-01), Griffiths et al.
patent: 6582915 (2003-06-01), Griffiths et al.
patent: 6593081 (2003-07-01), Grigg et al.
patent: 6916605 (2005-07-01), McCafferty et al.
patent: 7063943 (2006-06-01), McCafferty et al.
patent: 7195866 (2007-03-01), Griffiths et al.
patent: 2002/0150881 (2002-10-01), Ladner et al.
patent: B-27617/88 (1989-07-01), None
patent: 2 137 631 (1984-10-01), None
patent: 0 168 933 (1986-01-01), None
patent: A-0 368 684 (1990-05-01), None
patent: B-0 349 578 (1994-11-01), None
patent: 436597 (1997-04-01), None
patent: 9015198.6 (1990-07-01), None
patent: 9022845.3 (1990-10-01), None
patent: 9024503.6 (1990-11-01), None
patent: 91/25579 (1991-02-01), None
patent: 91/25582 (1991-02-01), None
patent: 9104744.9 (1991-03-01), None
patent: 9110549.4 (1991-05-01), None
patent: WO 83/02393 (1983-07-01), None
patent: WO 88/01649 (1988-03-01), None
patent: WO 88/06630 (1988-09-01), None
patent: 0 324 162 (1988-12-01), None
patent: WO 88/09344 (1988-12-01), None
patent: WO 90/02809 (1990-03-01), None
patent: WO 90/05144 (1990-05-01), None
patent: WO 90/14424 (1990-11-01), None
patent: WO 90/14430 (1990-11-01), None
patent: WO 90/14443 (1990-11-01), None
patent: WO 92-01047 (1992-01-01), None
patent: WO 92/06204 (1992-04-01), None
patent: WO 92/09690 (1992-06-01), None
patent: WO 92/18619 (1992-10-01), None
patent: WO 91/10737 (1997-07-01), None
patent: WO 91/17271 (1997-11-01), None
Huse et al., 1989, Generation of a large combinatorial library of the immunoglobulin repertoire in phage lambda, Science, 246(4935): 1275-1281.
Amit, A.G. et al., “Three-Dimentional Structure of an Antigen-Antibody Complex at 2.8 Å Resolution,”Science, 233:747-753 (Aug. 1986).
Armstrong, J. et al., “Domain Structure of Bacteriophage fd Adsorption Protein,”FEBS Lett., 135(1):167-172 (Nov. 1981).
Bass et al., “Hormone Phage: An Enrichment Method for Variant Proteins With Altered Binding Properties,”Proteins: Structure, Function and Genetics, 8:309-314 (1990).
Beck, E. et al., “Nucleotide sequence and genome organisation of filamentous bacteriophages fl and fd,”Gene, 16:35-58 (1981).
Beck, E. et al., “Nucleotide sequence of bacteriophage fd DNA,”Nucleic Acids Research, 5(12):4495-4503 (1978).
Berek, C. et al., “Molecular events during maturation of the immune response to oxazolone,”Nature, 316:412-418 (Aug. 1985).
Better, M. et al., “Escherichia coliSecretion of an Active Chimeric Antibody Fragment,”Science, 240:1041-1043 (May, 1988).
Bhat, T.N. et al., “Small rearrangements in structures of Fv and Fab fragments of antibody D1.3 on antigen bindings,”Nature, 347:483-485 (Oct. 1990).
Bird, R.E. et al., “Single chain antibody variable regions,”TIBTECH, 9:132-137 (Apr. 1991).
Bird, R.E. et al., “Single-Chain Antigen-Binding Proteins,”Science, 242:423-426 (Oct. 1988).
Boeke, J.D. et al., “Effects of Bacteriophage f1 Gene III Protein on the Host Cell Membrane,”Mol. Gen. Genet., 186:185-192 (1982).
Boyle, W., “An Extension of the51Cr-Release Assay for the Estimation of Mouse Cytotoxins,”Transplantation, 6(6):761-764 (1968).
Branden, C. et al., inIntroduction to Protein Structure, Garland Publishing, Inc., New York and London, pp. 26-29 (1991).
Brenna, O. et al., “Affinity-Chromatography Purification of Alkaline Phosphatase from Calf Intestine,”Biochem. J., 151:291-296 (1975).
Brownstone, A. et al., “Biological Studies with an Iodine-Containing Synthetic Immunological Determinant 4-Hydroxy-3-iodo-5-nitrophenylacetic Acid (NIP) and Related Compounds,”Immunology, 10:481-492 (1966).
Brownstone, A. et al., “Chemical and Serological Studies with an Iodine-Containing Synthetic Immunological Determinant 4-Hydroxy-3-iodo-5-nitrophenylacetic Acid (NIP) and Related Compounds,”Immunology, 10:465-479 (1966).
Carter, P. et al., “Improved oligonucleotide site-directed mutagenesis using M13 vectors”Nucl. Acids Res.,13(12):4431-4443 (1985).
Casali, P. et al., “Human Monoclonals from Antigen-Specific Selection of B Lymphocytes and Transformation by EBV,”Science, 234:476-479 (Oct. 1986).
Chaidaroglou, A. et al., “Function of Arginine-166 in the Active Site ofEscherichia coliAlakaline Phosphatase,”Biochemistry, 27:8338-8343 (1988).
Chang, C. N. et al., “Nuceotide sequence of the alkaline phosphatase gene ofEscherichia coli,” Gene, 44:121-125 (1986).
Chaudhary, V.K. et al., “A rapid method of cloning functional variable-region antibody genes inEscherichia colias single-chain immunotoxins,”Proc. Natl. Acad. Sci., USA, 87:1066-1070 (Feb. 1990).
Chiang, Y.L. et al., “Direct cDNA Cloning of the Rearranged Immunoglobulin Variable Region,”BioTechniques, 7(4):360-366 (1989).
Choo, Y. et al., “In vivo repression by a site-specific DNA-binding protein designed agai

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