Methods for producing L-isoleucine

Details Methods for producing L-isoleucine Methods for producing L-isoleucine

2006-01-17

2006-01-17

Ketter, James (Department: 1636)

Chemistry: molecular biology and microbiology

Micro-organism, per se ; compositions thereof; proces of...

C435S041000, C435S252100, C435S252300, C435S252320, C435S254100

Reexamination Certificate

active

06987017

ABSTRACT:
The present invention relates, in general, to the over-production of L-isoleucine by nonhuman organisms. More specifically, the present invention relates to methods for producing L-isoleucine comprising: (a) growing a transformed nonhuman organism under conditions that provide for synthesis of L-isoleucine, wherein the nonhuman organism comprises one or more copies of a transgene comprising at least one nucleotide sequence encoding catabolic threonine dehydratase; wherein the L-isoleucine is synthesized by the transformed nonhuman organism, the synthesis being greater than that of the corresponding non-transformed nonhuman organism; and (b) recovering the L-isoleucine from the culture medium in which the transformed nonhuman organism was cultured.

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