Methods for producing hyaluronan in a recombinant host cell

Details Methods for producing hyaluronan in a recombinant host cell Methods for producing hyaluronan in a recombinant host cell

2002-12-20

2010-10-12

Raghu, Ganapathirama (Department: 1652)

Chemistry: molecular biology and microbiology

Micro-organism, per se ; compositions thereof; proces of...

Bacteria or actinomycetales; media therefor

C435S471000, C435S320100, C435S069100, C435S101000, C435S084000

Reexamination Certificate

active

07811806

ABSTRACT:
The present invention relates to methods for producing a hyaluronic acid, comprising: (a) cultivating aBacillushost cell under conditions suitable for production of the hyaluronic acid, wherein theBacillushost cell comprises a nucleic acid construct comprising a hyaluronan synthase encoding sequence operably linked to a promoter sequence foreign to the hyaluronan synthase encoding sequence; and (b) recovering the hyaluronic acid from the cultivation medium. The present invention also relates to an isolated nucleic acid sequence encoding a hyaluronan synthase operon comprising a hyaluronan synthase gene and a UDP-glucose 6-dehydrogenase gene, and optionally one or more genes selected from the group consisting of a UDP-glucose pyrophosphorylase gene, UDP-N-acetylglucosamine pyrophosphorylase gene, and glucose-6-phosphate isomerase gene. The present invention also relates to isolated nucleic acid sequences encoding a UDP-glucose 6-dehydrogenase, UDP-glucose pyrophosphorylase, and UDP-N-acetylglucosamine pyrophosphorylase.

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