Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...
Patent
1995-06-05
1999-10-12
Degen, Nancy
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Recombinant dna technique included in method of making a...
435 711, 4351723, 435198, 4352543, C12P 2102, C12N 115, C12N 920, C12N 1580
Patent
active
059653841
ABSTRACT:
A process for expression of a protein product in Aspergillus oryzae is disclosed. The process comprises transforming Aspergillus oryzae with a vector system comprising DNA sequences encoding functions facilitating gene expression, a suitable marker for selection of transformants, and a DNA sequence encoding the desired protein product. The process enables industrial production of many different polypeptides and proteins in A. oryzae. Examples of such products are chymosin or prochymosin and other rennets, proteases, lipases and amylases. Also disclosed is an effective promoter for expression of a protein in Aspergillus. A preferred promoter is the TAKA-amylase promoter or functional parts thereof. There is also provided a process for the production of a recombinant Humicola lipase. The recombinant Humicola lipase from A. oryzae differs from the native lipase in having a greater glycosylation and in exhibiting an improved thermostability.
REFERENCES:
patent: 4486533 (1984-12-01), Lambowitz
patent: 4492758 (1985-01-01), Esser et al.
patent: 4588684 (1986-05-01), Brake et al.
patent: 4599311 (1986-07-01), Kawasaki
patent: 4666847 (1987-05-01), Alford et al.
patent: 4735901 (1988-04-01), Kurtz et al.
patent: 4810414 (1989-03-01), Huge-Jensen et al.
patent: 4816405 (1989-03-01), Timberlake et al.
patent: 4863864 (1989-09-01), Ashikari et al.
patent: 4885249 (1989-12-01), Buxton et al.
patent: 4885252 (1989-12-01), Ingolia et al.
patent: 5198345 (1993-03-01), Gwynne et al.
patent: 5364770 (1994-11-01), Berka et al.
patent: 5536661 (1996-07-01), Boel et al.
patent: 5578463 (1996-11-01), Berka et al.
Akins, R.A., et al. (1985) Mol. and Cell. Biol., 5, 2272-2278.
Alber et al. (1982) J. Mol. and Applied Genetics, 1, 419-434.
Alton, N.K., et al. (1982) Proc. Natl. Acad. Sci. USA, 79, 1955-1959.
Arends, H. et al. (1984) EMBO Journal, 3, 377-382.
Arima et al. (1972) Agric. Biol. Chem. 36(5), 893-95.
Ballance, D.J., et al. (1983) Biochem. and Biophys. Res. Comm., 112, 284-289.
Ballance, D.J., et al. (1985) Gene., 36, 321-331.
Ballance, D.J., et al. (1986) Mol. Gen. Genet., 202, 271-275.
Ballance et al. (1986) Yeast, 2, 229-236.
Ballance et al. (1986) "Sequences Important for Gene Expression in Filamentous Fungi", .sub.-- 2, 229.
Barnes, D.E., et al. (1986) Curr. Genet., 10, 767-775.
Bech, et al. (1981) Neth. Milk Dairy J., 35, 275-280.
Bennett (1985) Gene Manipulations in Fungi, Academic Press, 515-527.
Bennett (1985) Molecular Genetics of Filamentous Fungi, Alan R. Liss, Inc., 345-366.
Benoist et al. (1981) Nature 290, 304-310.
Beppu, T. (1983) Trends in Biotech., 1, 85-89.
Berse, B., et al. (1983) Gene, 25, 109-117.
Berka et al. ( ) "Molecular Cloning, Analysis and Expression of the gla1 Gene Encoding a Thermostable, Raw Starch-Digesting Glucoamylase from the Fungus Humicola grisea var. thermoidea".
Berka et al. (1989) Biotech, Adv., 7, 127-154.
Boel, E., et al. (1984) EMBO Journal, 3(7), 1581-1585.
Boel, E., et al. (1984) EMBO, J. 3(5): 1097-1102.
Boel, E., et al. (1984) Abstract P-26, EMBO Workshop, The Netherlands.
Briggs, M.S., et al. (1986). Adv. in Pro. Chem., 38, 109-180.
Bull, J.H., et al. (1984) Nature, 310, 701-704.
Burnett, "An Introduction to the Fungi", Chapter 1, Section 1--Structure and Growth, pp. 1-16.
Buxton, F.P., et al. (1983) Mol. Gen. Genet, 190, 403-405.
Buxton, F.P., et al. (1984) Mol. Gen. Genet, 196, 339-344.
Buxton, F.P., et al. (1985) Gene., 37, 207-214.
Caddick, M.X., et al. (1986) EMBO Journal, 5, 1087-1090.
Case, M.E., et al. (1979) Proc. Natl. Acad. Sci USA, 76, 5259-5263.
Chen et al. (1984) Acta Genet Sin, 11 (3), 159-170.
Cheng et al. (1990) Curr. Genet., 18, 453-456.
Christensen (1988) Biotechnology 6, 1419-1422.
Ciufetti et al. Molecular Biolog of Plant-Pathogen Interactions, p. 278, Abstract Y 305.
Cullen, D., et al. (1986) Heredity, 57, 131, Abstract No. 20.
Daboussi et al. (1989) Current Genetics, 15, 453-456.
Davies Leong (1990) "Molecular Biolgoy of a High-Level Recombinant Protein Production System in Aspergillus", Molecular Industry Mycology, Chptr. 3, pp. 45-81, Marcel Dekker, Inc.
Dhawale, S.S., et al. (1985) Current Genetics, 10, 205-212.
Doy, C.H., et al. (1985) DNA, 4, 105-114.
Durrens, P., et al. (1986) Mol. Gen. Genet., 203, 544-549.
Emtage, J.S., et al. (1983) Proc. Natl. Acad. Sci. USA, 80, 3671-3675.
Freer, S.N. (1983) Agr. Res. Ser., USDA, 175-193.
Goff, C.G., et al. (1984) Gene., 27, 35-46.
Goosen et al. (1984) Euro. Congr. Biotechn., 111-311-111-314.
Goosen et al. (1984) Abstract P-22, EMBO Workshop, The Netherlands.
Goosen et al. (1984) Abstract P-8, EMBO Workshop, The Netherlands.
Gotz et al. (1985) Neuclic Acids. Res. 13, 5895-5906.
Grant, D.M., et al. (1984) Mol. and Cell. Biol., 4, 2041-2051.
Grosveld et al. (1982) Nature 295, 120-126.
Gwynne et al. (1987) Biotechnology, 5, 713-719.
Hahm et al. (1990) Agric. Biol. Chem., 54, 25B-2520.
Hamer et al. (1987) Molecular and Cellular Biology, 7(7), 2352-2359.
Harris et al. (1982) Nucleic Acids Res. 10(7): 2177-2187.
Hawker et al. (1991) Gene, 100, 237-240.
Heyneker, H.L., et al. (1986) Proc. of Bio Expo., 86, 145-149.
Hidaka, M., et al. (1986) Gene., 43, 197-203.
Huiet, L., et al. (1986) Proc. Natl. Acad. Sci. USA, 83, 3381-3385.
Hynes, M.J., et al. (1983) Mol. and Cell. Biol., 3, 1430-1439.
Hynes, M.J. (1986) Experimental Mycology, 10, 1-8.
Iimura et al. (Mar. 10, 1986) Nihon Nogei Kagakukai 1986 Gists Lecture.
Iimura et al. (1987) Agricultural and Biological Chemistry 51, 323-28.
John et al. (1984) Enzyme Microb. Technol., 6, 386.
Johnstone (1984) Abstract P-30, EMBO Workshop, The Netherlands.
Johnstone, I.L. (1985) Microbiol. Sci. 2., 307-311.
Johnstone, I.L., et al. (1985) EMBO Journal, 4, 1307-1311.
Kawaguchi, Y., et al. (1984) Journal of Biotechnology, 1, 307-315.
Keesey, Jr., J.K., et al. (1982) Journal of Bacteriology, 152, 954-958.
Kelly, J.M., et al. (1985) EMBO Journal, 4, 475-479.
Kinnaird, J.H., et al. (1983) Gene, 26, 253-260.
Kinsey, J.A., et al. (1984) Mol. and Cell. Biol., 4, 117-122.
Kolar et al., "Molecular and Functional Analysis of the Promoter Region of the Penicillium Chryosogenum IPNS Gene", Abstract P18.
Kos et al. (1984) Abstract P-33 and Poster, EMBO Workshop, The Netherlands.
Kos, A., et al. (1985) Gene, 39, 231-238.
Krieg et al. (1984) J. Mol. Biol., 180: 615-640.
Kuck U. et al. Progress in Botany, 46, 201-204 (1984).
Kugimiya et al. (1986) Biochem. Biophys. Res. Comm., 141, 185-90.
Kuiper, M. T. R., et. al. Current Genetics, 9, 471-477.
Legerton, T. L., et. al. (1985) Gene, 39, 129-140.
Liang et al. (1982) Acta. Microbiol, Sin., 22(3), 248-256.
Liu et al (1973), Agr. Biol. Chem, 37(1), 157-163.
Marston, F.A.O., et al. (1984) Biotechnology, 2, 800-804.
Mattern et al. (1987) Mol. Gen. Genet., 210, pp. 460-461.
McCaman, M.T., et al. (1985) Journal of Biotechnology, 2, 177-190.
McGuire, J. (1986) Proc. of Bio Expo., 86, 121-141.
McKnight et al. (1981) Cell 25, 385-398.
McKnight et al. (1982) Science 217, 316-324.
McKnight, G.L. (1985) J. Cell. Biochem. Suppl., 9C, 137.
McKnight, G.L., et al. (1986) Cell, 46, 143-147.
Mellor, J., et al. (1983) Gene., 24, 1-14.
Miller, B.L., et al. (1985) Mol. and Cell Biol., 5, 1714-1721.
Minoda et al. (1969) Agric. Biol. Chem., 33(4). 572-578.
Moir, D.T., et al., (1982) Gene, 19, 127-138.
Moir et al. (1983) Chemical Abstracts, vol. 98, No. 15, 173, Abstract 120575m.
Moir, D.T., et al. (1985) Developments in Microbiology, 26, 75-85.
Mullaney, E.J., et al. (1985) Mol. Gen. Genet., 199, 37-45.
Munger, K., et al. (1985) EMBO Journal, 4, 2665-2668.
Nishimori, K., et al. (1981) J. Biochem., 90, 901-904.
Nishimori, K., et al. (1982) J. Biochem., 91, 1085-1088.
Nishimori, K., et al. (1982) Gene, 19, 337-344.
Nishimori, K., et al. (1984) Gene, 29, 41-49.
Nunberg, J.A., et al. (1984) Mol. and Cell. Biol., 4, 2306-2315.
Ohnuki et al. (1982) Agric. Biol. Chem., 46(2), 451-458.
Orbach, M.J., et al. (1986) Moll. & Cell. Biol., 6, 2452-2461.
Paietta, J., et al. (1985) Current Genetics, 9, 383-388.
Penttilla, M.E., et al. (1984) Mol. Gen. Genet, 194, 494-499.
Punt et al. (1987) Gene, 56, 117.
Rutledge, B.J. (1984) Gene, 32, 275-287.
Samson,
Boel Esper
Christensen Tove
Huge-Jensen Ida Birgitte
Woldike Helle Fabricius
Degen Nancy
Lambiris Elias J.
Novo Nordisk A S
Zelson Steve T.
LandOfFree
Methods for producing Humicola lipases in aspergillus does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Methods for producing Humicola lipases in aspergillus, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Methods for producing Humicola lipases in aspergillus will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-651128