Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Patent
1997-03-13
1999-02-09
Scheiner, Toni R.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
4353913, 53038815, 5303888, 5303913, 5303917, 436 64, G01N 33574, C07K 1618
Patent
active
058692681
ABSTRACT:
A method for producing a human lymphocyte producing an antibody specific to a desired antigen, a method for producing a monoclonal antibody, as well as monoclonal antibodies produced by the method, are described. The present invention provides a method for producing a human lymphocyte producing an antibody comprising, in the order mentioned, the steps of: transplanting human lymphocytes into an immunodeficient animal so that the human lymphocytes take in the animal body; immunizing the animal with a desired antigen so as to generate human lymphocytes producing an antibody specific to the antigen; and recovering the human lymphocytes producing the antibody from the animal. The present invention also provides a method for producing a monoclonal antibody comprising, in the order mentioned, the steps of: immortalizing the human lymphocytes producing the antibody which were obtained by the above-mentioned method of the present invention; cloning the obtained immortalized human-derived lymphocytes producing the antibody; and recovering a monoclonal antibody specific to the desired antigen from the cloned immortalized human-derived lymphocytes.
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Hiyamuta Shuichi
Kadota Akihiko
Kudo Toshio
Tanedani Toshiyuki
Idemitsu Kosan Company Limited
Scheiner Toni R.
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