Chemistry: molecular biology and microbiology – Treatment of micro-organisms or enzymes with electrical or... – Modification of viruses
Patent
1992-10-30
1997-10-28
Scheiner, Toni R.
Chemistry: molecular biology and microbiology
Treatment of micro-organisms or enzymes with electrical or...
Modification of viruses
435 723, 43524027, 53038815, 5303888, 53038885, G01N 33574, C12N 1507
Patent
active
056817290
ABSTRACT:
A method for isolating a human lymphocyte producing an antibody specific to a desired antigen, a method for isolating a monoclonal antibody, as well as monoclonal antibodies produced by the method, are described. The present invention provides a method for isolating a human lymphocyte producing an antibody comprising, in the order mentioned, the steps of: transplanting human lymphocytes into an immunodeficient animal so that the human lymphocytes take in the animal body; immunizing the animal with a desired antigen so as to generate human lymphocytes producing an antibody specific to the antigen; recovering lymphocytes producing antibody by incubating with anti-mouse antibody; and separating human lymphocytes from mouse by centrifugation. The present invention also provides a method for producing a monoclonal antibody comprising, in the order mentioned, the steps of: immortalizing the human lymphocytes producing the antibody which were obtained by the above-mentioned method of the present invention; cloning the obtained immortalized human-derived lymphocytes producing the antibody; and recovering a monoclonal antibody specific to the desired antigen from the cloned immortalized human-derived lymphocytes.
REFERENCES:
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Hiyamuta Shuichi
Kadota Akihiko
Kudo Toshio
Tanedani Toshiyuki
Idemitsu Kosan Company Limited
Scheiner Toni R.
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