Methods for producing gamma-carboxylated proteins

Details Methods for producing gamma-carboxylated proteins Methods for producing gamma-carboxylated proteins

2004-10-14

2010-11-30

Ketter, James S (Department: 1636)

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Recombinant dna technique included in method of making a...

C514S008100, C536S024100

Reexamination Certificate

active

07842477

ABSTRACT:
The present invention relates to methods and tools for producing large quantities of gamma-carboxylated protein comprising: (i) culturing a cell adapted to express a protein which requires gamma-carboxylation and γ-glutamyl carboxylase in a ratio of at least 10:1, under conditions suitable for expression of both proteins, and (ii) isolating gamma-carboxylated protein.

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