Methods for producing and transforming cassava protoplasts

Organic compounds -- part of the class 532-570 series – Organic compounds – Carbohydrates or derivatives

Reexamination Certificate

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C536S045000, C536S055300, C536S102000, C536S124000, C536S127000, C536S128000, C435S421000

Reexamination Certificate

active

06982327

ABSTRACT:
The invention relates to a method for producing protoplasts of cassava or closely related species, which protoplasts are capable of regeneration into plants. The method comprises producing friable embryogenic callus from explants of cassava or closely related species and isolating protoplasts from said friable embryogenic callus. The invention also concerns protoplasts obtainable by said method. The invention further relates to a method for transforming such a protoplast of cassava or closely related species, and transformed protoplasts obtainable thereby. In addition, the invention concerns a method for regenerating plants from these protoplasts and a cassava plant or closely related species obtainable thereby.Starch isolated from tubers of such cassava plants have an increased amylopectin content. The starch and a method for isolating it from said plants also forms part of the invention.

REFERENCES:
patent: 3890888 (1975-06-01), Verberne
patent: 4285735 (1981-08-01), Mitchell et al.
patent: 5349123 (1994-09-01), Shewmaker et al.
patent: 5824798 (1998-10-01), Tallberg et al.
patent: 6207880 (2001-03-01), Kossmann et al.
patent: WO 93/10255 (1993-05-01), None

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