Methods for producing agarose gels having variable pore sizes

Details Methods for producing agarose gels having variable pore sizes Methods for producing agarose gels having variable pore sizes

1989-09-22

1991-04-23

Niebling, John F.

Chemistry: electrical and wave energy

Processes and products

Electrostatic field or electrical discharge

204299R, 2041801, G01N 2726, B01D 5702

Patent

active

050097598

ABSTRACT:
Provided are agarose gels and processes for producing agarose gels having a uniform concentration of agarose and a pore size gradient. These gels are prepared by allowing gelation in a salt or buffer gradient. The agarose gels are suitable for the purposes of sieving based electrophoretic separations or other sieving-based separations, such as, chromatography.

REFERENCES:
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Rodbard, D. et al., Anal. Biochem., vol. 40 (1971), 135-157.
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Fawcett, J. S. et al., Electrophoresis, vol. 10 (1989), 182-185.
Waki, S. et al., Biopolymers, vol. 21 (1982), 1909-1926.
Peats, S. et al., Biophysical Journal, vol. 49 (1986), 91a.
Griess, G. A. et al., Biopolymers, vol. 28, (1989), 1-10.
Sheen, J.-Y. et al, Biotechniques, vol. 6 (1988), 942-944.

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