Methods for producing a polypeptide in a Bacillus cell

Details Methods for producing a polypeptide in a Bacillus cell Methods for producing a polypeptide in a Bacillus cell

2007-02-20

2007-02-20

Monshipouri, Maryam (Department: 1653)

Chemistry: molecular biology and microbiology

Micro-organism, per se ; compositions thereof; proces of...

Bacteria or actinomycetales; media therefor

C435S069100, C435S320100, C435S252300, C435S201000, C435S207000

Reexamination Certificate

active

09834271

ABSTRACT:
The present invention relates to methods for producing a polypeptide, comprising: (a) cultivating aBacillushost cell in a medium conducive for the production of the polypeptide, wherein theBacilluscell comprises a nucleic acid construct comprising (i) a tandem promoter in which each promoter sequence of the tandem promoter is operably linked to a single copy of a nucleic acid sequence encoding the polypeptide and alternatively also (ii) an mRNA processing/stabilizing sequence located downstream of the tandem promoter and upstream of the nucleic acid sequence encoding the polypeptide; and (b) isolating the polypeptide from the cultivation medium. The present invention also relates to methods for producing a polypeptide, comprising: (a) cultivating aBacillushost cell in a medium conducive for the production of the polypeptide, wherein theBacilluscell comprises a nucleic acid construct comprising (i) a “consensus” promoter having the sequence TTGACA for the “−35” region and TATAAT for the “−10” region operably linked to a single copy of a nucleic acid sequence encoding the polypeptide and (ii) an mRNA processing/stabilizing sequence located downstream of the “consensus” promoter and upstream of the nucleic acid sequence encoding the polypeptide; and (b) isolating the polypeptide from the cultivation medium.

REFERENCES:
patent: 93/10249 (1993-05-01), None
patent: 94/25612 (1994-11-01), None
patent: WO 94/25612 (1994-11-01), None
patent: 96/23073 (1996-08-01), None
Hung et al., Mol. Gen. Genet., vol. 219, 129-136, 1989.
Diderichsen et al., Res. Microbiol., 142, 7-8, 793-96, 1991.
Aqaisse et al., Molecular Microbiology, 13(1), pp. 97-107 (1994).
Hue et al., Journal of Bacteriology, vol. 177, no. 12, pp. 3465-3471 (1995).
Ichikawa et al., FEMS Microbiology Letters, 111, pp. 219-224, (1993).

Affiliated with

Also associated with

Rating

Methods for producing a polypeptide in a Bacillus cell does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Methods for producing a polypeptide in a Bacillus cell, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Methods for producing a polypeptide in a Bacillus cell will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-3889483