Chemistry: molecular biology and microbiology – Virus or bacteriophage – except for viral vector or...
Patent
1997-03-28
1998-08-11
Ketter, James
Chemistry: molecular biology and microbiology
Virus or bacteriophage, except for viral vector or...
435260, 4353201, C12N 104
Patent
active
057926433
ABSTRACT:
Methods for preserving an infectious recombinant virus for subsequent reconstitution are provided. Within one aspect, the method comprises the steps of (a) combining an infectious recombinant virus with an aqueous solution comprising a saccharide, a high molecular weight structural additive, a buffering component and water to form an aqueous suspension, thereby stabilizing the infectious virus; (b) cooling the aqueous suspension containing the virus to a temperature below the glass transition state temperature or below the eutectic point temperature of the formulation; and (c) removing water from the cooled aqueous suspension by sublimation to form a lyophilized virus having less than 10% water by weight of the lyophilized virus, the virus being capable of infecting mammalian cells upon reconstitution.
REFERENCES:
patent: 3915794 (1975-10-01), Zygraica et al.
patent: 4132775 (1979-01-01), Volenec et al.
patent: 4338335 (1982-07-01), McAleer et al.
patent: 4380582 (1983-04-01), Orlando et al.
patent: 4500512 (1985-02-01), Barme
patent: 4859587 (1989-08-01), Roizman
Moritsugu Freeze-Dried Hepatitis A Vaccine Containg Amino Acids and Sugars as Stabilizers, Chem Abstracts 113(2): 12106d, p. 369, col. 2 (1990).
Rowe, Plaque Assay Techniques for Murine Leukemia Viruses, Virology 42: 1136-39(1970).
Rowe, Machinery and methods in Freeze-Drying, Cyrobiology 8: 153-72(1971).
Rightsel, Freezing and Freeze-Drying of Viruses, Cryobiology 3:423-31(1967).
Phillips, A Study of Water Binding in Lyophilized Viral Vaccine Systems, Cryobiology 18: 414-19(1981).
Stamp, The Preservation of Bacteria by Drying, J Gen Microbiol 1: 251-65(1947).
Flosdorf, Procedure and Appartus for Preservation in Lyophile form of Serum and Other Biological Substances, J Immunol. 29: 389-425 (1935).
Sitch, PCT International Search Report for PCT/US94/11414, Jan. 25, 1995, 4 pages total.
Berge et. al. (1971) Appl. Microbiol. 22(5) 850-853.
Levy et. al. (1982) J. Virol. Meth. 5, 165-171.
Webster's II: New Riverside. University Dictionary (1988) Houghton Mifflin Co. Boston MA. pp. 646 & 956.
Herrmann Steven M.
Prussak Charles E.
Blackburn Robert P.
Cserr Luann
Ketter James
Kruse Norman J.
Yucel Irem
LandOfFree
Methods for preserving recombinant retroviruses does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Methods for preserving recombinant retroviruses, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Methods for preserving recombinant retroviruses will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-388146