Methods for preparing polynucleotides encoding orphan receptor l

Details Methods for preparing polynucleotides encoding orphan receptor l Methods for preparing polynucleotides encoding orphan receptor l

1995-06-15

1998-01-06

Walsh, Stephen

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving antigen-antibody binding, specific binding protein...

435 72, 435 721, 435 6, 435 691, 435 695, 4351721, 435372, 4353721, 435405, 436501, 536 231, 536 235, C12N 1512, C12N 1519

Patent

active

057053499

ABSTRACT:
Methods for obtaining cells that produce a ligand for an orphan receptor and methods for preparing polynucleotide molecules that encode ligands for orphan receptors are disclosed. The methods utilize growth factor-dependent parent cells that are transfected with a DNA construct encoding an orphan receptor. The transfected cells are exposed to mutagenizing conditions, and the mutagenized cells are cultured under conditions in which cell survival is dependent upon autocrine growth factor production. Progeny cells are recovered and screened to identify those that produce a ligand for the orphan receptor. Polynucleotide molecules encoding the ligand can be prepared from the identified cells.

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