Methods for preparing nucleic acid samples

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing compound containing saccharide radical

Reexamination Certificate

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C435S091210

Reexamination Certificate

active

07432084

ABSTRACT:
In one aspect the present invention provides methods of synthesizing a preparation of nucleic acid molecules, the methods comprising the steps of: (a) utilizing an RNA template to enzymatically synthesize a first DNA molecule that is complementary to at least 50 contiguous bases of the RNA template; (b) utilizing the first DNA molecule as a template to enzymatically synthesize a second DNA molecule, thereby forming a double-stranded DNA molecule wherein the first DNA molecule is hybridized to the second DNA molecule; (c) utilizing the first or second DNA molecule of the double-stranded DNA molecule as a template to enzymatically synthesize a first RNA molecule that is complementary to either the first DNA molecule or to the second DNA molecule; and (d) utilizing the first RNA molecule as a template to enzymatically synthesize a third DNA molecule that is complementary to the first RNA molecule. In another aspect, the present invention provides processed DNA samples prepared by a method of the invention for synthesizing a preparation of nucleic acid molecules. In another aspect, the present invention provides methods for hybridizing a processed DNA sample to a population of immobilized nucleic acid molecules.

REFERENCES:
patent: 5409818 (1995-04-01), Davey et al.
patent: 5665545 (1997-09-01), Malek et al.
patent: 5914229 (1999-06-01), Loewy
patent: 6261770 (2001-07-01), Warthoe
patent: 6271002 (2001-08-01), Linsley et al.
patent: 6794141 (2004-09-01), Erlander et al.
patent: WO 9708185 (1997-03-01), None
patent: WO 9955378 (1999-04-01), None
Buck et al. Biotechniques, vol. 27, pp. 528-536, Sep. 1999.

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