Methods for preparation and use of a coomassie brilliant blue/pr

Details Methods for preparation and use of a coomassie brilliant blue/pr Methods for preparation and use of a coomassie brilliant blue/pr

1998-08-05

2000-05-02

Ceperley, Mary E.

Chemistry: analytical and immunological testing

Peptide, protein or amino acid

436501, 436815, 5303911, 5303913, 530403, G01N 33483, C07K 113

Patent

active

060571608

ABSTRACT:
A rapid and simple method for making a hydrophobically associated, Coomassie Brilliant Blue (CBB) dye/protein complex comprising CBB and a protein, and a method for detecting analytes using the CBB dye/protein complex are disclosed. The method for making the CBB dye/protein complex is comprised of mixing a protein with a non-alcoholic aqueous CBB solution having a pH greater than 4, heating the mixture to a first temperature for a short pulse period, then cooling to a second temperature to form a solution containing the CBB dye/protein complex. In the method for detecting analytes that bind a CBB dye/protein complex, the analyte sample is added to the solution containing a CBB dye/protein complex to form a supernatant and a precipitate containing an analyte-bound fraction. The precipitate is dissolved in an assay solvent, and a spectral absorption of both the precipitate and supernatant is measured.

REFERENCES:
Martin et al, Oecologia, vol. 54, 205-211 (1982).
Fazekas de St. Groth et al, Biochem. Biophys. Acta, vol. 71, 377-391 (1963).
Compton et al, Analytical Biochemistry, vol. 151, 369-374 (1985).
Atherton et al, Anal. Biochem., vol. 233, 160-168 (1996).
Bradford, Anal. Biochem., vol. 72, 248-254 (1976).
Asquith et al., J. Chem. Ecology, vol. 11, 1535-1544 (1985).

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