Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing compound containing saccharide radical
Patent
1998-02-03
2000-07-04
Fredman, Jeffrey
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Preparing compound containing saccharide radical
435 6, 435 912, 435 9121, 536 231, 536 243, 536 2431, 536 2432, 536 2433, C12P 1934, C12Q 168, C07H 2102
Patent
active
060837268
ABSTRACT:
This invention relates to methods for making polynucleotides of any desired nucleotide sequence by repeating a series of reactions involving assembly of overlapping oligomers, ligation by DNA ligase, and cleavage with a restriction enzyme that cuts so as to add one or more additional nucleotides to a growing polynucleotide. This invention also relates to combining such a method for polynucleotide synthesis with a step employing localized melting of hybridized DNA oligomers, to synthesize an array of polynucleotides of different, defined nucleotide sequence on a substrate, and to the articles for polynucleotide hybridization so made. This invention further includes methods in which a substrate bearing an array of polynucleotides made according to the invention is used to determine the nucleotide sequence of a nucleic acid, or to detect or isolate a nucleic acid having a selected nucleotide sequence, and a device which analyzes a substrate-bound array of polynucleotides made according to the invention to determine the nucleotide sequence of a nucleic acid, or to detect or isolate a nucleic acid having a selected nucleotide sequence.
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Mills, Jr. Allen P.
Yurke Bernard
Fredman Jeffrey
Lucent Technologies - Inc.
Schneller John W.
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