Multicellular living organisms and unmodified parts thereof and – Method of introducing a polynucleotide molecule into or... – Via agrobacterium
Reexamination Certificate
2005-08-16
2011-11-29
Fox, David T (Department: 1638)
Multicellular living organisms and unmodified parts thereof and
Method of introducing a polynucleotide molecule into or...
Via agrobacterium
C800S279000, C800S300000, C800S302000, C800S303000, C435S430000, C435S431000
Reexamination Certificate
active
08067673
ABSTRACT:
The present description concerns methods for regeneration of whole plant from the explants obtained from theAbelmoschusspecies preferablyA. esculentus. In addition the present description also concerns methods for transforming okra plant, plant cells and tissues either with the use of recombinantAgrobacteriumstrain or by bombarding the explants with tungsten or gold particles coated with DNA sequences of interest. An efficient method to isolate embryos from imbibed seeds of okra is also described which enables the use of young meristematic cells of plumule tip for efficient regeneration and transformation of okra plants. Further, transformed okra plants, plant cells and tissues for improved agronomic
on agronomic traits and insect resistance are produced either by using marker based or marker free systems.
REFERENCES:
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Deole Satish Govindrao
Nair Madhavan Narendran
Zehr Usha Barwale
Fox David T
Lowe Hauptman & Ham & Berner, LLP
Maharashtra Hybrid Seeeds Company Ltd.
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