Chemistry: physical processes – Physical processes – Crystallization
Patent
1977-12-13
1980-11-04
Marantz, Sidney
Chemistry: physical processes
Physical processes
Crystallization
23915, 424 8, 424 12, 435 4, 250459, G01N 3316, G01N 3114, G01N 2122
Patent
active
042317509
ABSTRACT:
Improved methods for determining very low concentrations of substances present in fluid samples are provided by employing light emitting tracer compounds and (1) counting the photons emitted therefrom while discriminating against noise, nonspecific light, and quenching effects of the sample, or (2) counting the photons emitted therefrom over a predetermined integrated light flux, or a combination of (1) and (2). Further, novel fluorescently labeled low molecular weight antigens are provided which can be employed in competitive binding techniques in which the above described photon counting methods are useful. A homogeneous competitive binding assay, employing photon emitting tracer materials, which eliminates the need for separating bound from unbound materials is also provided. Finally, a modified enzyme amplification technique is set forth employing enzymes active in the bound phase to provide assay techniques useful for extremely low concentration assays.
REFERENCES:
patent: 3453288 (1969-07-01), McConnell
patent: 3586859 (1971-06-01), Katz
patent: 3641235 (1972-02-01), Rozman
patent: 3654090 (1972-04-01), Schuurs
patent: 3741876 (1973-06-01), Guilbault
patent: 3789116 (1974-01-01), Kay
patent: 3826613 (1974-07-01), Parikh
patent: 3852157 (1974-12-01), Rubenstein
patent: 3853987 (1974-12-01), Dreyer
patent: 3854050 (1974-12-01), Peterson
patent: 3875011 (1975-04-01), Rubenstein
patent: 3879262 (1975-04-01), Schuurs
patent: 3901654 (1975-08-01), Gross
patent: 3915641 (1975-10-01), Goering
patent: 3925162 (1975-12-01), Kanno
patent: 3940475 (1976-02-01), Gross
patent: 3998943 (1976-12-01), Ullman
patent: 4014745 (1977-03-01), Fletcher
patent: 4016043 (1977-04-01), Schuurs
patent: 4020151 (1977-04-01), Bolz
patent: 4062935 (1977-12-01), Masson
patent: 4104029 (1978-08-01), Maier
patent: 4133639 (1979-01-01), Harte
M. L. Franklin et al., Anal. Chem., 41(1), 2-10 (1969).
K. C. Ash et al., Anal. Chem., 43(1), 26-34 (1971).
J. D. Ingle et al., Anal. Chem., 44(4), 785-794 (1972).
H. V. Malmstadt et al., Anal. Chem., 44(8), 63A, 64A, 66A, 68A, 71A, 72A, 74A, 76A (1972).
J. A. R. Mead et al., Biochem Jour., 61(4), 569-574 (Dec. 1955).
R. H. Schneider et al., Rev. Sci. Instruments, 39(9), 1369-1371, Sep. 1968.
"Emit, The Non-Radioactive Immunoassay," Booklet by Syva Corp.
Bunting James R.
Dowben Robert M.
Diagnostic Reagents, Inc.
Marantz Sidney
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