Chemistry: analytical and immunological testing – Involving an insoluble carrier for immobilizing immunochemicals
Patent
1991-01-18
1993-03-30
Kepplinger, Ester L.
Chemistry: analytical and immunological testing
Involving an insoluble carrier for immobilizing immunochemicals
422 56, 422 58, 435810, 436527, 436528, 436178, 436180, 436805, 436807, 436809, 436810, G01N 33543, G01N 33546
Patent
active
051983680
ABSTRACT:
A method for performing a microparticle diagnostic assay in a device having a shallow sample well for receiving a sample and reagents for forming a reaction mixture, a read well positioned adjacent to said sample well and separated from said sample well by a wall which is constructed and arranged such that when wash fluid is injected into said sample well, sample and reaction mixtures are washed from said shallow sample well, over the wall and into said read well. The steps of the method comprise forming microparticle analyte complexes in said shallow sample well; washing said microparticle analyte complexes from said shallow sample well over the wall and into said read well where a fibrous matrix retains and immobilizes said microparticle analyte complexes; adding to said read well an indicator substance capable of forming an assay signal when said microparticle analyte complexes are present; and detecting said assay signal produced by said indicator substance.
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Hanna Charles F.
Khalil Omar S.
Zurek Thomas F.
Abbott Laboratories
Chin Christopher L.
Kepplinger Ester L.
Porembski Priscilla E.
Ungemach Frank s.
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