Methods for obtaining nucleic acid containing a mutation

Details Methods for obtaining nucleic acid containing a mutation Methods for obtaining nucleic acid containing a mutation

1998-11-18

2000-03-07

Ketter, James

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 911, 536 241, 536 254, C12Q 168

Patent

active

06033861&

ABSTRACT:
In order to facilitate the screening of an organism, or a population of organisms, carrying heterozygous mutations for identifying the presence of a mutation in a gene of interest, a method is provided which utilizes mismatch binding proteins, such as MutS. The method comprises the steps of denaturing double stranded nucleic acid present in a nucleic acid sample from an organism; allowing the nucleic acid to anneal; and removing homoduplexes from the annealed sample, thereby retaining heteroduplexes in the sample. A positive signal from a probe specific for the gene of interest indicates that the organism carries a mutation in the gene.

REFERENCES:
Casna et al., Nuc. Acids Res., vol. 14, 1986, 7285-7303.

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