Methods for nucleic acid detection, sequencing, and cloning usin

Details Methods for nucleic acid detection, sequencing, and cloning usin Methods for nucleic acid detection, sequencing, and cloning usin

1995-06-07

1997-11-18

Degen, Nancy

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing compound containing saccharide radical

435 6, 4351723, C12P 1934, C12N 1510, C12N 1566, C12Q 168

Patent

active

056886691

ABSTRACT:
The present invention provides a method of detecting the presence of a nucleotide sequence within a double-stranded DNA in a sample comprising: a. digesting the double-stranded DNA with an exonuclease which converts at least a portion of the double-stranded DNA to single-stranded DNA, b. binding the single-stranded DNA with a nucleic acid probe which selectively hybridizes with the single-stranded DNA, and c. detecting hybridization between the single-stranded DNA and the nucleic acid probe, the existence of hybridization indicating the presence of the nucleotide sequence within the double-stranded DNA in the sample. The present invention further provides a method of detecting the presence of a nucleotide sequence in a sample comprising DNA which is the product of a DNA amplification technique. The invention also provides methods of sequencing and cloning using exonuclease.

REFERENCES:
patent: 4683195 (1987-07-01), Mullis et al.
patent: 5516633 (1996-05-01), Fuller
patent: 5518901 (1996-05-01), Murtagh
Gyllensten et al. (1993). Methods in Enzymology 218:3-16.
Ward et al. (1989), Nucl. Acids Res. 17(20):8394.
White TJ, Madej, R, Persing DH. Adv. Clin. Chem. 1992;29-161-96.
Persing DH. J. Clin. Microbiol. 1991;29:1281-5.
Jung V, Pestka SB, Pestka S. Nucleic Acid Res 18:6156.
Zhang Y, Coyne MY, Will SG, Levenson CH, Kawasaki ES. Nucleic Acids Res. 1991;19:3929-33.
Helmuth R. Nonisotopic detection of PCR products. In: Innis MA, Gelfand DH, Sninsky JJ, White TJ (eds.), PCR Protocols: A Guide to Methods and Applications. San Diego, CA: Acedemic Press, 1990;119-28.
Gyllensten UB, Erlich HA. Proc Natl Acad Sci USA 1988;85:7652.
Stoker AW. Nucleic Acids Res 1990; 13:4290.
Aslanidis C, de Jong P. Nucleic Acids Res 1990;18:6069-74.
Kuijper Gene 1992;112:147-55.
Haun RS et al. BioTechniques 1992;13:515-8.
Haun R, Moss J. Gene 1992;112:37-43.
Pham T, Cheng QL. Clontechques 1993;8:5-9.
Lindahl T, Ljungquist S, Siegert W, Nyberg B, Sperens B. J. Biol Chem 1977;252:3286-94.
Rastchian A, Buchman GW, Schuster DM, Berninger MS. Anal Biochem 1992;206:91-7.
Rashtchian A, Thornton CG, Heidecker G. PCR Methods Appl 1992;2:124-30.
Nisson PE, Rashtchian A, Watkins PC. PCR Methods Appl 1991;1:120-3.
Wahlberg et al. Proc Natl Acad Sci USA 1990;87:6569-73.
Drake T, Hindler JA, Barton GW, Bruckner DA. J Clin Microbiol 1987;25:1442-5.
Yamane et al. Nucleic Acids Symp. Ser., 1988; 19:93-95.

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