Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing compound containing saccharide radical
Patent
1993-03-10
1994-05-24
Chambers, Scott A.
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Preparing compound containing saccharide radical
435 6, 536 243, C12Q 168, C12P 1934, C07H 1512
Patent
active
053148096
ABSTRACT:
Methods are provided for enhanced specificity and sensitivity of nucleic acid amplification. The methods are simplified nested amplification procedures wherein both inner and outer primer pairs are present in the amplification reaction mixture. According to the methods, the thermocycling profile, as well as the sequences, length, and concentration of amplification primers, is modified to regulate which primers are annealed and extended on the target during any particular amplification cycle. The methods described are particularly suitable in PCR amplifications and have numerous applications in molecular biology, medical diagnostics, and forensics.
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Erlich Henry A.
Higuchi Russell G.
Chambers Scott A.
Gould George M.
Hoffman-La Roche Inc.
Rocha Patricia S.
Sias Stacey R.
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