Plant husbandry – Process
Patent
1981-10-14
1984-01-17
Feyrer, James R.
Plant husbandry
Process
47DIG1, A01G 100
Patent
active
044257376
ABSTRACT:
A method is disclosed which selects for different types of mutants expressed in cereal seed. The method allows rapid qualitative or quantitative screening of the aleurone GA.sub.3 -induced expression of alpha-amylase activity in half-seeds (without embryo). Half-seeds of the M.sub.2 population of mutagenized cereal seeds are incubated under selection pressures that normally inhibit the expression of alpha-amylase activity. These selection agents include metabolic inhibitors which may function at the level of transcription, translation, or protein secretion.
These selection agents include antimetabolites such as amino acid analogs (e.g., S-2-aminoethylcysteine, p-flourophenylalaine, canavanine, etc.), antibiotics (e.g. Actinomycin D, cycloheximide, erythromycin, kanamycin, streptomycin, or tunicamycin), biocides, herbicides, water or salt stress. Such selection pressures may be applied to the half-seed method in the presence of absence of GA.sub.3, ABA, and at various incubation temperatures. Once the half-seeds which produce alpha-amylase in the presence of the selected antimetabolite are identified, the corresponding embryo-containing portion of the seed may be germinated and grown to maturity.
REFERENCES:
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Agrigenetics Research Associates Limited
Feyrer James R.
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