Chemistry: molecular biology and microbiology – Animal cell – per se ; composition thereof; process of... – Method of regulating cell metabolism or physiology
Patent
1996-11-15
1999-10-05
Low, Christopher S. F.
Chemistry: molecular biology and microbiology
Animal cell, per se ; composition thereof; process of...
Method of regulating cell metabolism or physiology
514 2, 514 12, 514144, 536 235, 4353201, 435455, 435456, 435458, 435471, 435325, 435361, 4352351, 935 9, 935 32, 935 33, 935 34, 935 52, 935 57, 935 70, 935 71, 424 91, 424 92, C12N 506, C12N 510
Patent
active
059623225
ABSTRACT:
Methods for regulation of lipid and cholesterol uptake are described which are based on regulation of the expression or function of the SR-BI HDL receptor. The examples demonstrate that estrogen dramatically downregulates SR-BI under conditions of tremendous upregulation of the LDL-receptor. The examples also demonstrate the upregulation of SR-BI in rat adrenal membranes and other non-placental steroidogenic tissues from animals treated with estrogen, but not in other non-placental non-steroidogenic tissues, including lung, liver, and skin. Examples further demonstrate the uptake of fluorescently labeled HDL into the liver cells of animal, which does not occur when the animals are treated with estrogen. Examples also demonstrate the in vivo effects of SR-BI expression on HDL metabolism, in mice transiently overexpressing hepatic SR-BI following recombinant adenovirus infection. Overexpression of the SR-BI in the hepatic tissue caused a dramatic decrease in cholesterol blood levels. These results demonstrate that modulation of SR-BI levels, either directly or indirectly, can be used to modulate levels of cholesterol in the blood.
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Kozarsky Karen
Krieger Monty
Rigotti Attilio
Low Christopher S. F.
Massachusetts Institute of Technology
The Trustees of the University of Pennsylvania
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