Methods for modulating protein function in cells using intracell

Details Methods for modulating protein function in cells using intracell Methods for modulating protein function in cells using intracell

1996-06-24

2000-02-22

Campell, Bruce R.

Drug, bio-affecting and body treating compositions

Designated organic active ingredient containing

Carbohydrate doai

A61K 4800

Patent

active

06028059&

ABSTRACT:
Methods and compositions for modulating protein function in a cell involving intracellular expression of an antibody homologue that binds to the protein within the cell are disclosed. In a preferred embodiment, an antibody homologue, such as a single chain Fv (sFv) fragment, is expressed within an intracellular compartment of a cell, such as the endoplasmic reticulum (ER), to inhibit cell surface expression of a membrane protein. Preferably, the cell is a malignant mammalian cell and the protein is a cell surface receptor oncoprotein, such as erbB2. Intracellular binding of the antibody homologue to the receptor oncoprotein inhibits its surface expression and, moreover, inhibits cell proliferation and cell survival. Isolated nucleic acid molecules encoding anti-c-erbB2 antibody homologues, as well as recombinant expression ventors and host cells incorporating these nucleic acid molecules, are also disclosed.

REFERENCES:
JG Altin et al (1991) Growth Factors 4: 145-155 (abstract only).
JR Starkey et al (1991) FASEB J 5: A1259.
JA Drebin et al (1986) Proc Natl Acad Sci USA 83: 9129-9133.
IM Harwerth et al (1993) Br J Cancer 68: 1140-1145.
J Deshane et al (1994) Gene Therapy 1: 332-337.
Werge et al FEBS 274 (1,2): 193, 1990.
Wels et al Cancer Res 52: 6310, 1992.
Wels et al J Steroid Biochem. Mol. Biol 43(1-3): 1, 1992.
Brocea et al BBRC 197(2): 422, 1993.
Marasco et al PNAS 90: 7889, 1993.
R. Hesketh (1995) The Oncogene Facts Book pp. 32-42.

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