Methods for modifying the production of a polypeptide

Details Methods for modifying the production of a polypeptide Methods for modifying the production of a polypeptide

1997-09-12

1999-09-28

Ketter, James

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Recombinant dna technique included in method of making a...

435 711, 435 712, 435440, 435455, 435471, 435325, 4352523, 43525411, 4352543, 4352544, 4352546, 4352547, 4352548, C12N 1500

Patent

active

059587270

ABSTRACT:
The present invention relates to methods for modifying the production of a polypeptide, comprising: (a) introducing a nucleic acid construct into a cell, wherein the cell comprises a DNA sequence encoding a polypeptide, under conditions in which the nucleic acid construct integrates into the genome of the cell at a locus not within the DNA sequence encoding the polypeptide to produce a mutant cell, wherein the integration of the nucleic acid construct modifies the production of the polypeptide by the mutant cell relative to the cell when the mutant cell and the cell are cultured under the same conditions; and (b) identifying the mutant cell with the modified production of the polypeptide.

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patent: 5272071 (1993-12-01), Chappel
patent: 5578461 (1996-11-01), Sherwin et al.
patent: 5641670 (1997-06-01), Treco et al.
Berkner, K.L. Current Topics in Microbiology. vol. 158, pp. 39-60, 1992.
Gordon et al. Current Opinion in Biotechnology. vol. 5, pp. 611-616, 1994.
Kay et al. Science. vol. 262, pp. 117-119, 1993.

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