Methods for manufacturing hydrogen using anaerobic digestion

Liquid purification or separation – Processes – Treatment by living organism

Reexamination Certificate

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C210S613000, C210S631000

Reexamination Certificate

active

07540961

ABSTRACT:
A method for manufacturing a biomass enriched with hydrogen-producing bacteria or spores includes providing a biomass comprising a hydrogen-producing bacteria and a competing bacteria and treating the biomass with a sufficient amount of a chemical agent for a period of time such that the treatment (i) kills, inhibits or injures substantially all of the competing bacteria and (ii) does not kill or inhibit the hydrogen-producing bacteria or causes the hydrogen-producing bacteria to form spores that are not destroyed during the chemical treatment. The method also includes digesting an enriched biomass with a non-sterile organic substrate. The enriched biomass includes hydrogen-producing bacteria or spores that are mixed in sufficient quantities with the organic substrate such that the hydrogen-producing bacteria can overcome the competing bacteria to consume the organic substrate and produce hydrogen.

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M. Okamoto, T. Miyahara, O. Mizuno and T. Noike; Biological hydrogen potential of materials characteristic of the organic fraction of municipal solid wastes; Water Science and Technology, vol. 41 No. 3 pp. 25-32 IWA Publishing 2000.
Jiunn-Jyi Lay; Modeling and Optimization of Anaerobic Digested Sludge converting Starch to Hydrogen; Biotechnology and Bioengineering, vol. 68, No. 3, pp. 269-278 May 5, 2000.
Peter Setlow; Resistance of Bacterial Spores; Bacterial Stress Responses, Department of Biochemistry, University of Connecticut Health Center, Farmington, CT 06032, Chapter 14, pp. 217-230 2000 ASM Press, Washington D.C.

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