Methods for making nucleic acids

Details Methods for making nucleic acids Methods for making nucleic acids

1998-03-27

2000-09-05

Elliott, George C.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing compound containing saccharide radical

C12P 1934

Patent

active

061141526

ABSTRACT:
Nucleic acids are made by adding a known nucleotide sequence to the 3' end of a first RNA having a known sequence at the 5' end to form a second RNA and reverse transcribing the second RNA to form a cDNA. In one embodiment, the first RNA is an amplified mRNA, the known sequence at the 5' end comprises a poly(T) sequence, the adding step comprises using a polyadenyltransferase to add a poly(A) sequence to the 3' end, the reverse transcribing step is initiated at a duplex region comprising the poly(T) sequence hybridized to the poly(A) sequence, the cDNA is converted to double-stranded cDNA by a polymerase initiating from a noncovalently joined duplex region, and the double-stranded cDNA is transcribed to form one or more third RNAs.

REFERENCES:
patent: 4952496 (1990-08-01), Studier et al.
patent: 5514545 (1996-05-01), Eberwine
Lin et al., NAR 29 (21) : 4954-4960, 1993.
Brady et al, Methods in Mol. & Cell. Biol. 2:17-25, 1990.
Toellner KM et al.: "The use of reverse transcription polymerase chain reaction to analyse large numbers of mRNA species from a single cell" Journal of Immunological Methods, vol. 191, 1996, pp. 71-75.

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