Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1995-06-07
1998-09-29
Jones, W. Gary
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
435 912, 435 911, 536 243, 536 2431, 536 2433, C12Q 168, C12P 1934, C07H 2104
Patent
active
058144447
ABSTRACT:
Methods for making chromosome-specific libraries from single chromosomes are disclosed in which a single flow-sorted chromosome (or subchromosomal fragment) is efficiently collected, and DNA extracted from the chromosome is amplified, e.g., by PCR. After they are produced, the resulting libraries are screened, e.g., by in situ hybridization or hybridization to a chromosome spot blot, to identify libraries that arise from the chromosome (or subchromosomal fragment) of interest. The single-chromosome amplification libraries and individual DNA sequences from the libraries are useful, for example, for cytogenetic analysis and cancer diagnostics.
REFERENCES:
patent: 5427932 (1995-06-01), Weier et al.
patent: 5461907 (1995-10-01), Tench et al.
patent: 5491224 (1996-02-01), Bittner et al.
Marder et al, "Selective cloning of hybridoma cells for enhanced immunoglobulin production using flow cytometric cell sorting and automated laser nephelometry", Cytometry 11:498-505, 1990.
Djabali et al, "Laser microdissection of the fragile.times.region: Identification of cosmid clones and of conserved sequences in this region", Genomics 10: 1053-1060, 1991.
VanDevanter et al., "Pure Chromosome-Specific PCR Libraries From Single Sorted Chromosomes," Proc. Natl. Acad. Sci. USA 91:5858-5862 (Jun. 1994).
Bohlander et al., "A Method for the Rapid Sequence-Independent Amplification of Microdissected Chromosomal Material," Genomics 13:1322-1324 (1992).
Boschman et al., "Identification of a Tumor Marker Chromosome by Flow Sorting, DNA Amplification In Vitro, and In Situ Hybridization of the Amplified Product," Genes, Chromosomes & Cancer 6:10-16 (1993).
Carter et al., "Reverse Chromosome Painting: a Method for the Rapid Analysis of Aberrant Chromosomes in Clinical Cytogenetics," J. Med. Genet. 29:299-307 (1992).
Cox et al., "Radiation Hybrid Mapping: A Somatic Cell Genetic Method for Constructing High-Resolution Maps of Mammalian Chromosomes," Science 250:245-250 (1990).
Cram, "Flow Cytogenetics and Chromosome Sorting," Human Cell 3:99-106 (1990).
Don et al., "`Touchdown` PCR to Circumvent Spurious Priming During Gene Amplification," Nucleic Acids Res. 19:4008 (1991).
Gray et al., "Flow Karyotyping and Chromosome Sorting," in Flow Cytometry and Sorting, eds. Melamed et al., WileyLiss, New York, pp. 503-529 (1990).
Jinno et al., "A Simple and Efficient Amplification Method of DNA with Unknown Sequences and its Application to Microdissection/Microcloning," J. Biochem. 112:75-80 (1992).
Kallioniemi et al., "Comparative Genomic Hybridization for Molecular Cytogenetic Analysis of Solid Tumors," Science 258:818-821 (1992).
Langford et al., "Chromosome Painting Using Chromosome-Specific Probes from Flow-Sorted Pig Chromosomes," Cytogenet. Cell Genet. 61:221-223 (1992).
Lebo et al., "High-Resolution Chromosome Sorting and DNA Spot-Blot Analysis Assign McArdle's Syndrome to Chromosome 11," Science 225:57-59 (1984).
Lengauer et al., "Chromosomal Bar Codes Produced by Multicolor Fluorescence In Situ Hybridization with Multiple YAC Clones and Whole Chromosome Painting Probes," Hum. Mol. Genet. 2:505-512 (1993).
Lisitsyn et al., "Cloning the Differences Between Two Complex Genomes," Science 259:946-951 (1993).
Meltzer et al., "Rapid Generation of Region Specific Probes by Chromosome Microdissection and Their Application," Nature Genetics 1:24-28 (1992).
Chang et al., "PCR Amplification of Chromosome-Specific DNA Isolated from Flow Cytometry-Sorted Chromosomes," Genomics 12:307-312 (1992).
Ostrander et al., "Construction of Small-Insert Genomic DNA Libraries Highly Enriched for Microsatellite Repeat Sequences," Proc. Natl. Acad. Sci. USA 89:3419-3423 (1992).
Pinkel et al., "Fluorescence In Situ Hybridization with Human Chromosome-Specific Libraries: Detection of Trisomy 21 and Translocations of Chromosome 4," Proc. Natl. Acad. Sci. USA 85:9138-9142 (1988).
Suijkerbuijk et al., "Fluorescent In Situ Identification of Human Marker Chromosomes Using Flow Sorting and Alu Element-Mediated PCR," Genomics 13:355-362 (1992).
Telenius et al., "Cytogenetic Analysis by Chromosome Painting Using DOP-PCR Amplified Flow-Sorted Chromosomes," Genes, Chromosomes & Cancer 4:257-263 (1992).
VanDevanter et al., "Recombination Between Separate MYC Amplification Structures in COLO320 Cells," Genes, Chromosomes & Cancer 6:190-197 (1993).
Van Dilla et al., "The National Laboratory Gene Library Project," in Gray, ed., Flow Cytogenetics, Academic Press, New York, pp. 257-274 (1989).
Vooijis et al., "Libraries for Each Human Chromosome, Constructed from Sorter-Enriched Chromosomes by Using Linker-Adaptor PCR," Am. J. Hum. Genet. 52:586-597 (1993).
Wienberg et al., "Molecular Cytotaxonomy of Primates by Chromosomal In Situ Suppression Hybridization," Genomics 8:347-350 (1990).
Wienberg et al., "Homologies in Human and Macaca fuscata Chromosomes Revealed by In Situ Suppression Hybridization with Human Chromosome Specific DNA Libraries," Chromosoma 101:265-270 (1992).
Fredman Jeffrey
Jones W. Gary
University of Washington
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