Methods for maize transformation coupled with adventitious regen

Multicellular living organisms and unmodified parts thereof and – Method of introducing a polynucleotide molecule into or... – Involving particle-mediated transfecion

Patent

Rate now

  [ 0.00 ] – not rated yet Voters 0   Comments 0

Details

800278, 435420, 435424, 435430, 435431, 435470, A01H 400, A01H 100, C12N 504, C12N 1582, C12N 1587

Patent

active

061405558

ABSTRACT:
Maize tissue may be regenerated from nodal extracts prepared from germinated mature seeds and germinated embryos. Nodal section explants are secured from seedlings in 3-5 days. The explants are grown on an induction medium until adventitious shoot formation is observed. The shoots are separated and elongated on an MS-based medium, and then rooted. Fast genotype-independent regeneration is obtained, in 12-14 weeks. These explants, as well as zygotic embryos, may be transformed with exogenous DNA using a biolistic approach, where DNA precipitated onto tungsten microprojectiles is accelerated as 650 psi towards the explants at a distance of at least 7.5 microns. Improved frequency of transformation is obtained using microprojectiles which prior to DNA precipitation were frozen in glycerol, and suspending from a preparation of 2.5 M CaCl.sub.2. The combination of transformation process and regeneration can be used, independent of genotype, to provide new commercial crop organisms.

REFERENCES:
patent: 5164310 (1992-11-01), Smith et al.
patent: 5583210 (1996-12-01), Neill et al.
patent: 5736369 (1998-04-01), Bowen et al.
Hartmann et al, Plant Science; Growth, Development, and Utilization of Cultivated Plants, Prentice-Hall, Inc., Englewood Cliffs, New Jersey, p. 18, 1981.
Schwarz and Beaty, Chapter eleven: Propagation from Nonmeristematic Tissues--Organogenesis, CRC Press, pp. 95-104, 1996.
Douglas, G.C., Chapte 8: Manipulation of Shoot Formation in Cultured Explants, Methods in Molecular Biology, vol. 6, Plant Cell and Tissue Culture, The Humana Press, pp. 71-79, 1990.
Ritchie et al, How a Corn Plant Develops: Special Report No. 48, Iowa State University of Science and Technology, pp. 1-21, 1986.
Pierik, In Vitro Culture of Higher Plant, Martinus Nijhoff Publishers, pp. 1-6, 291, 1987.
Pareddy et al, Maize Transformation via Helium Blasting, Maydica 42:143-151, 1997.
Brown and Thorpe, Chapter 2: Plant Regeneration by Organogenesis, Cell Culture and Somatic Cell Genetics of Plants, vol. 3, Academic Press, Inc., pp. 49-65, 1986.
Dodds and Roberts, Experiments in Plant Tissue Culture, chapter 6: Organogenesis, Cambridge University Press, pp. 82-91, 1995.
Dodds and Roberts, Experiments in Plant Tissue Culture, chapter 10, Micropropagation by bud proliferation, Cambridge University Press, pp. 126-135, 1995.
Southgate et al, A Comparison of Methods for Direct Gene Transfer into Maize (Zea mays L.), In Vitro Cell. Dev. Biol.--Plant 34:218-224, 1998.
Raven et al., Biology of Plants, sixth edition, W.H. Freeman and Company Worth Publishers, p. 611, 1999.
Levetin and McMahon, Plants and Society, McGraw-Hill, pp. 43-44, 1981.
Biotechnology, vol. 11, pp. 596-598, May, 1993, Dennis E. McCabe, et al., "Transformation of Elite Cotton Cultivars Via Particle Bombardment of Meristems".
Plant Cell Reports, 8:586-589, (1990), John J. Finter, et al., "Transformation of Cotton (Gossypium hirsutum L.) Via Particle Bombardment".
Plant Molecular Biology Reporter, 13 (1) 1995, pp. 31-37, Caryl A Chlan, et al., "A Procedure for Biolistic Transformation and Regeneration of Transgenic Cotton from Meristematic Tissue".
Plant Molecular Biology 10:105-116 (1987), Ebrahim Firoozabady, et al., "Transformation of Cotton (Gossypium hirsutum L.) by Agrobacterium Tumefaciens and Regeneration of Transgenic Plants".
In Vitro Cell. Dev. Biol. 29 P:166-173, Oct., 1993, E. Firoozabady, et al., "Plant Regeneration via Somatic Embryogenesis in Many Cultivars of Cotton (Gossypium hirsutum L.)".
Critical Reviews in Biotechnology, 17(3): 185-208 (1997), Mmaliyakal E. John, "Cotton Crop Improvement Through Genetic Engineering".
Plant Cell Reports (1997) 16:647-652, D.C. Agrawal, et al., "In Vitro Induction of Multiple Shoots and Plant Regeneration in Cotton (Gossypium hirsutum L.)".

LandOfFree

Say what you really think

Search LandOfFree.com for the USA inventors and patents. Rate them and share your experience with other people.

Rating

Methods for maize transformation coupled with adventitious regen does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Methods for maize transformation coupled with adventitious regen, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Methods for maize transformation coupled with adventitious regen will most certainly appreciate the feedback.

Rate now

     

Profile ID: LFUS-PAI-O-2054115

  Search
All data on this website is collected from public sources. Our data reflects the most accurate information available at the time of publication.