Chemistry: analytical and immunological testing – Involving an insoluble carrier for immobilizing immunochemicals – Carrier is inorganic
Patent
1997-06-19
2000-01-11
Eisenschenk, Frank C.
Chemistry: analytical and immunological testing
Involving an insoluble carrier for immobilizing immunochemicals
Carrier is inorganic
436501, 436518, 436524, 436536, 435 79, 435 792, G01N 33553, G01N 33566, G01N 33543, G01N 33536
Patent
active
060135328
ABSTRACT:
Microbiological entities, such as cells or microbes, are immobilized in a single-file linear array for optical analysis. Colloidal magnetic particles having a binding agent, such as ligand for attachment with a corresponding receptor, are bound to the entities suspended in a fluid medium. The fluid medium is placed into a vessel having a ferromagnetic capture structure including an elongated linear collection surface with a diameter less than that of the microbiological species of interest. The vessel is placed into a magnetic field for inducing a magnetic gradient in a region along the collection surface of the ferromagnetic capture structure. The magnetically-labeled entities are attracted toward the collection surface and immobilized thereon in a linear array. Microscopic optical and fluorescence observations, and sequential chemical reactions and mechanical manipulations may be performed on the line of immobilized entities. Methods of analysis for blood and immune cells, such as leukocytes and lymphocytes, are disclosed.
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Liberti Paul A.
Wang Yuzhou
Eisenschenk Frank C.
Immunivest Corporation
Nelson Brett
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