Methods for lyophilizing competent cells

Details Methods for lyophilizing competent cells Methods for lyophilizing competent cells

2005-11-01

2005-11-01

Afremova, Vera (Department: 1651)

Chemistry: molecular biology and microbiology

Micro-organism, per se ; compositions thereof; proces of...

Bacteria or actinomycetales; media therefor

C435S252800, C435S260000, C435S849000, C424S093400, C424S093480

Reexamination Certificate

active

06960464

ABSTRACT:
This invention relates to a method for producing cells which are competent for transformation and which may be stably stored for extended periods of time at various temperatures. The method involves growing cells in a growth conducive medium, rendering said cells competent, and lyophilizing said competent cells. The invention further relates to competent cells produced by such a method, to methods of transforming said cells with a DNA molecule, and to a method of producing a desired protein or polypeptide from said transformed cells.

REFERENCES:
patent: 3843453 (1974-10-01), Freake
patent: 4038143 (1977-07-01), Juni
patent: 4446230 (1984-05-01), Zubrzycki
patent: 4520019 (1985-05-01), Ribi et al.
patent: 4681852 (1987-07-01), Tribe
patent: 4808404 (1989-02-01), Bhogal
patent: 4824938 (1989-04-01), Koyama et al.
patent: 4851348 (1989-07-01), Hanahan
patent: 4891319 (1990-01-01), Roser
patent: 4950609 (1990-08-01), Tischer et al.
patent: 4981797 (1991-01-01), Jessee et al.
patent: 5043261 (1991-08-01), Goodrich et al.
patent: 5045446 (1991-09-01), Goodrich, Jr. et al.
patent: 5059518 (1991-10-01), Kortright et al.
patent: 5098893 (1992-03-01), Franks et al.
patent: 5149656 (1992-09-01), Bitton et al.
patent: 5153004 (1992-10-01), Goodrich, Jr. et al.
patent: 5178884 (1993-01-01), Goodrich et al.
patent: 5213814 (1993-05-01), Goodrich, Jr. et al.
patent: 5292507 (1994-03-01), Charley
patent: 5425951 (1995-06-01), Goodrich, Jr. et al.
patent: 5891692 (1999-04-01), Bloom et al.
patent: 5958670 (1999-09-01), Goodrich, Jr. et al.
patent: 6274369 (2001-08-01), Donahue, Jr. et al.
patent: 6383810 (2002-05-01), Fike et al.
patent: 2001/0046698 (2001-11-01), Donahue, Jr. et al.
patent: A-27434/88 (1989-06-01), None
patent: 0 383 569 (1990-08-01), None
patent: 0 508 496 (1992-10-01), None
patent: WO 97/28248 (1997-08-01), None
patent: WO 97/36613 (1997-10-01), None
Anderson, D.M.W. and I.C.M. Dea, “Recent advances in the chemistry ofAcaciagums,”J. Soc. Cosmet. Chem. 22:61-76, Society of Cosmetic Chemists of Great Britain (1971).
Chung, C.T., et al., “One-step preparation of competentEscherichia coli: Transformation and storage of bacterial cells in the same solution,”Proc. Natl. Acad. Sci. USA 86:2172-2175, National Academy of Sciences (1989).
Cohen, S.N., et al., “Nonchromosomal Antibiotic Resistance in Bacteria: Genetic Transformation ofEscherichia coliby R-Factor DNA,”Proc. Natl. Acad. Sci. USA 69:2110-2114, National Academy of Sciences (1972).
Cosloy, S.D. and M. Oishi, “Genetic Transformation inEscherichia coliK12,”Proc. Natl. Acad. Sci. USA 70:84-87, National Academy of Sciences (1973).
Crowe, J.H., et al., “Stabilization of dry phospholipid bilayers and proteins by sugars,”Biochem. J. 242:1-10, The Biochemical Society (1987).
Dagert, M. and S.D. Ehrlich, “Prolonged Incubation in Calcium Chloride Improves the Competence ofEscherichia coliCells,”Gene 6:23-28, Elsevier/North-Holland Biomedical Press (1979).
Grant & Hackh's chemical dictionary, Grant, R.L., ed., McGraw-Hill, Inc., pp. 246, 346 (1987).
Green, J.L. and C.A. Angell, “Phase Relations and Vitrification in Saccharide-Water Solutions and the Trehalose Anomaly,”J. Phys. Chem. 93:2880-2882, the American Chemical Society (1989).
Hanahan, D., “Studies on Transformation ofEscherichia coliwith Plasmids,”J. Mol. Biol. 166:557-580, Academic Press, Inc. (1983).
Hatley, R.H.M., et al., “The Stabilization of Labile Biochemicals by Undercooling,”Process Biochem. 22:169-172. Morgan-Grampain (1987).
Hatley, R.H.M. and F. Franks, “Variation in Apparent Enzyme Activity in Two-Enzyme Assay Systems: Phosphoenolpyruvate Carboxylase and Malate Dehydrogenase,”Biotechnol. &Appl. Biochem. 11:367-370, Academic Press, Inc. (1989).
Heckly, R.J. and J. Quay, “A Brief Review of Lyophilization Damage and Repair in Bacterial Preparations,”Cryobiology 18:592-597, Academic Press, Inc. (1981).
Kushner, S.R., “An Improved Method for Transformation ofEscherichia Coliwith ColEl Derived Plasmids,” In:Genetic Engineering, Boyer, H.W., and S. Nicosia, eds., Elsevier/North-Holland Biomedical Press, New York, NY, pp. 17-23 (1978).
Liu, H. and A. Rashidbaigi, “Comparison of Various Competent Cell Preparation Methods for High Efficiency DNA Transformation,”BioTechniques 8:21-25, Eaton Publishing Group (1990).
Mandel, M. and A. Higa, “Calcium-dependent Bacteriophage DNA Infection,”J. Mol. Biol. 53:159-162, Academic Press, Inc. (1970).
Merck Index: An Encyclopedia of Chemicals, Drugs, and Biologicals, 11th edition, Budavari, S. et al., eds., Merck & Co., Inc., Rahway, NJ, pub., p. 3 (1989).
Polisky, B., et al., “Specifically of substrate recognition by the EcoRI restriction endonuclease,”Proc. Natl. Acad. Sci. USA 72:3310-3314, National Academy of Sciences (1975).
Sambrook, J. et al., “Plasmid Vectors: Preparation and Transformation of CompetentE. coli,” In:Molecular Cloning: A Laboratory Manual, 2nd Ed., Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York, pp. 1.74-1.84 (1989).
Simione, Jr., F.P., “Key Issues Relating to the Genetic Stability and Preservation of Cells and Cell Banks,”J. Parenteral Sci.&Technol. 46:226-232, Parenteral Science and Technology (1992).
Tang, X. et al., “The optimization of preparations of competent cells for transformation ofE. coli,” Nucl. Acids Res. 22:2857-2858, Oxford University Press (1994).
Weisburd, S., “Death-Defying Dehydration,”Science News 133:107-110, Science Service (1988).
Life Technologies, Inc. 1993-94 Catalogue and Reference Guide, GIBCO BRL, pp. 6-10 and 9-4 (1993).
American Type Culture Collection,ATCC Bacteria and Bacteriophages, 19th Ed., American Type Culture Collection, pp. 152-153, 161, 188, 277, 320 (1996).
Gherna, R.L., “Chapter 12. Preservation,” in:Manual of Methods for General Bacteriology, Gerhardt, P., et al., eds., American Society for Microbiology, Washington, DC, pp. 208-217, (1981).
Hermann, C.H. and Rice, A.P., “Specific Interaction of the Human Immunodeficiency Virus Tat Proteins with a Cellular Protein Kinase,”Virol. 197:601-608, Academic Press, Inc., (1993).
Invitrogen Corporation Catalog, “Competent Cells,” Invitrogen Corporation, pp. 71-72, (1992).
Leslie, S.B., et al., “Trehalose and Sucrose Protect Both Membranes and Proteins in Intact Bacteria during Drying,”Appl. Envir. Micro. 61:3592-3597, American Society for Microbiology (1995).
Life Technologies, Inc., 1995-1996 Catalogue and Reference Guide, GIBCO BRL, pp. 16.2-16.12 (1995).
MacLachlan, P.R. and Sanderson, K.E., “Transformation ofSalmonella typhimuriumwith Plasmid DNA: Differences Between Rough and Smooth Strains,”J. Bacter. 161:442-445, American Society for Microbiology (1985).
Stratagene, “Competent Cells and Packaging Extracts,”Stratagene, pp. 63-74, Stratagene (1995).
Alexander, D.C., et al., “A simplified and efficient vector-primer cDNA cloning system,”Gene 31:79-89, Elsevier Science Publishers (1984).
Bullock, W.O., et al., “XL1-Blue: A High Efficiency Plasmid Transforming recAEscherichia coliStrain With Beta-Galactosidase Selection,”BioTechniques 5:376-378, Eaton Publishing Group (1987).
Cronan, Jr., J.E., “Thermal Regulation of the Membrane Lipid Composition ofEscherichia coli,” J. Biol. Chem. 250:7074-7077, the American Society of Biological Chemists, Inc. (1975).
de Mendoza, D., et al., “Overproduction of cis -Vaccenic Acid and Altered Temperature Control of Fatty Acid Synthesis in a Mutant ofEscherichia coli,”J. Bacteriol. 151:1608-1611, American Society for Microbiology (1982).
de Mendoza, D., et al., “Thermal Regulation of Membrane Fluidity inEscherichia coli,” J. Biol. Chem. 258:2098-2101, the American Society of Biological Chemists, Inc. (1983).
de Mendoza, D. and J.E. Cronan, Jr

Affiliated with

Also associated with

Rating

Methods for lyophilizing competent cells does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Methods for lyophilizing competent cells, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Methods for lyophilizing competent cells will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-3518918