Multicellular living organisms and unmodified parts thereof and – Method of using a plant or plant part in a breeding process... – Method of breeding maize
Reexamination Certificate
2011-04-19
2011-04-19
Ibrahim, Medina A. (Department: 1638)
Multicellular living organisms and unmodified parts thereof and
Method of using a plant or plant part in a breeding process...
Method of breeding maize
C800S260000, C800S320100, C800S298000
Reexamination Certificate
active
07928287
ABSTRACT:
The present invention provides for rapid and large scale evaluation of expression of, or function of, nucleotide sequences in plants. The invention comprises three specific components which provide for fast and large scale evaluation of nucleotide sequences. The first component includes delivery in either a single event a library of different engineered vectors or a single engineered vector for a single target nucleotide sequence comprising sequences the function of which is desired to be known in plant cells. Surprisingly, applicants have discovered that, the introduction of multiple vectors to plant cells predominantly results in individual transgenic plants which contain only a single transformation event. The second feature of the invention involves a highly transformable, fast cycling and/or miniature size plant and the final step involves mass scale analysis of T0 plants for various phenotypes and plasmid rescue to identify the nucleotide sequence present in a particular phenotype.
REFERENCES:
patent: 5981840 (1999-11-01), Zhao et al.
Koester et al. 1993. Crop Sci. 33: 1209-1216.
Zhao et al. 2001. Molecular Breeding 8: 323-333.
Koester et al. 1993. Crop Sci. 33: 1209-1216.
Niu Xiaomu
Oliveira Igor C.
Tomes Dwight T.
Wang Haiyin
Xu Deping
Ibrahim Medina A.
McKee Voorhees & Sease, P.L.C.
Pioneer Hi-Bred International , Inc.
Robinson Keith O.
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