Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Reissue Patent
1997-10-08
2008-10-28
Sisson, Bradley L. (Department: 1634)
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
C435S091100, C435S091200, C436S501000, C436S094000, C436S800000, C530S350000, C536S023100, C536S024300, C536S025300
Reissue Patent
active
RE040557
ABSTRACT:
The present invention provides improved methods for labeling nucleotides. The method of the invention for labeling nucleotides comprises the steps of: reacting a reactive moiety of a linker, which linker is a platinum compound having a stabilizing bridge and two reactive moieties, with an electron donating moiety of a spacer, which spacer comprises a chain having at least four atoms and at least one heteroatom in the chain, which spacer further comprises said electron donating moiety at one end of the chain and a reactive moiety at the other end of the chain; reacting the reactive moiety of said spacer with a label; reacting the other reactive moiety of said linker with a nucleotide. A major advantage of the invention is that all nucleotides can be labeled by the method of the invention, whereas until now the attachment of a label was mostly restricted to one or certain nucleotides.
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Heetebrij Robert Jochem
Houthoff Jan Hendrick
Jelsma Tinka
Reedijk Jan
Volkers Herman Hendricus
Hoffmann & Baron , LLP
Kreatech Biotechnology B.V.
Sisson Bradley L.
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