Methods for labeling intracytoplasmic molecules

Details Methods for labeling intracytoplasmic molecules Methods for labeling intracytoplasmic molecules

1995-02-14

1997-07-15

Spiegel, Carol A.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving antigen-antibody binding, specific binding protein...

435 72, 435 725, 435 405, 435962, 435968, 436 8, 436 66, 436174, 436176, 436548, 436825, C12N 500, G01N 3353

Patent

active

056482205

ABSTRACT:
The invention features a method of labeling a cell containing an intracytoplasmic target molecule involving (1) permeabilizing the plasma membrane of the cell so that (a) a reagent capable of detectably labeling the intracytoplasmic target molecule can traverse the plasma membrane into the cytoplasm of the cell; and (b) substantially all of the intracytoplasmic target molecule and the DNA of the cell remain in the cell; and (2) contacting the cell with the reagent to label the intracytoplasmic target molecule. The method may further involve detecting the label in the cell, and isolating the cell on the basis of detecting the label in the cell. The invention also includes cells permeabilized using the method of the invention.

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