Methods for isolation and labeling of sample molecules

Details Methods for isolation and labeling of sample molecules Methods for isolation and labeling of sample molecules

2007-02-27

2007-02-27

Ceperley, Mary E. (Department: 1641)

Chemistry: analytical and immunological testing

Peptide, protein or amino acid

C435S006120, C436S071000, C436S172000, C436S173000, C436S501000, C530S402000, C530S405000

Reexamination Certificate

active

09858198

ABSTRACT:
The invention provides methods for labeling a molecule by contacting a sample molecule with a solid support coupled to a chemical group comprising a cleavable functional group, one or more functional groups, and a reactive group for the sample molecule, under conditions allowing the sample molecule to covalently bind to the reactive group; and cleaving the cleavable functional group, thereby releasing the sample molecule comprising the one or more functional groups, which can be a tag. The invention also provides a solid support covalently coupled to a chemical group comprising a cleavable functional group, a mass spectrometry tag and a reactive group for covalently attaching a sample molecule, wherein the cleavable functional group, the tag and the reactive group are positioned relative to each other to allow transfer of the tag to the sample molecule upon cleavage of the cleavable functional group.

REFERENCES:
patent: 6057096 (2000-05-01), Rothschild et al.
patent: WO 99/02728 (1999-01-01), None
patent: WO 00/11208 (2000-03-01), None
patent: WO 01/96869 (2001-12-01), None
patent: WO 02/48717 (2002-06-01), None
Bodanszky and BodanszkyThe Practice of Peptide Synthesis, vol. 21 Springer-Verlag, New York (1984), Table of Contents only.
Brancia et al., “A combination of chemical derivatisation and improved bioinformatic tools optimises protein identification for proteomics,”Electrophoresis22:552-559 (2001).
Glazer et al., “Laboratory Techniques in Biochemistry and Molecular Biology: Chemical Modification of Proteins,”Elsevier Biomedical Press, New York Chapter 3, pp. 68-120 (1975).
Gygi et al., “Evaluation of two-dimensional gel electrophoresis-based proteome analysis technology,”Proc. Natl. Acad. Sci. USA97:9390-9395 (2000).
Gygi et al., “Quantitative analysis of complex protein mixtures using isotope-coded affinity tags,”Nature Biotechnol. 17:994-999 (1999).
Houghten, R. “General method for the rapid solid-phase synthesis of large numbers of peptides: specificity of antigen-antibody interaction at the level of individual amino acides,”Proc. Natl. Acad. Sci. USA, 82:5131-5135 (1985).
Hoving et al., “A method for the chemical generation of N-terminal peptide sequence tags for rapid protein identification,”Anal. Chem. 72:1006-1014 (2000).
Merrifield, R.B. “Solid Phase Peptide Synthesis,”J. Am. Chem. Soc. 85:2149-2154 (1963).
Munchbach et al., “Quantitation and Facilitated de Novo Sequencing of Proteins by Isotopic N-Terminal Labeling of Peptides with a Fragmentation-Directing Moiety,”Anal. Chem. 72:4047-4057 (2000).
Wilson and Czarnik, eds.,Combinatorial Chemistry: Synthesis and Application, John Wiley & Sons, New York (1997). Table of Contents only.
Zhou et al., “A systematic approach to the analysis of protein phosphorylation,”Nature Biotechnol. 19:375-378 (2001).
Aebersold et al., “Mass spectrometry in proteomics,”Chem. Reviews101(2):269-295 (2001).
Geysen et al., “Isotope or mass encoding of combinatorial libraries,”Chem. Biol. 3:679-688 (1996).
Zhou et al., “Quantitative proteome analysis by solid-phase isotope tagging and mass spectrometry,”Nature Biotech. 19:512-515 (2002).

Affiliated with

Also associated with

Rating

Methods for isolation and labeling of sample molecules does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Methods for isolation and labeling of sample molecules, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Methods for isolation and labeling of sample molecules will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-3837001