Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Enzymatic production of a protein or polypeptide
Reexamination Certificate
2011-03-29
2011-03-29
Lilling, Herbert J. (Department: 1657)
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Enzymatic production of a protein or polypeptide
C435S004000, C530S327000, C530S344000
Reexamination Certificate
active
07915008
ABSTRACT:
Changes in sialylation of cell surface or plasma proteins are often associated with various cancers and other disease conditions. Provided are methods of detecting biomarkers of conditions associated with a change of sialylation status. Sialylated peptides are first isolated from biological and other samples by loading onto titanium dioxide (TiO2) or zirconium dioxide (ZrO2) stationary phase under acidic conditions in a solution comprising at least 20% organic phase and at least about 6.5 mM of substituted aromatic carboxylic acid, or, alternatively, at least about 11 mM short chain, non-aromatic, hydroxylated carboxylic acid. Sialic acid containing proteins can then be eluted from loaded stationary phase material by exposure to an alkaline solution having pH of 9.0 or greater, preferably at least 10.5. Sialylated peptides isolated by the methods provided can then be analysed by mass spectrometry to identify patterns of sialylation across a sialiome (the entire complement of sialic acid containing peptides in a biological sample) and/or to identify proteins in a sample that are sialylated or that show changes in sialylation status between two or more different samples. In preferred embodiments, sialylated peptides from control and patient samples can be differentially isotopically labelled and compared in a single mass sprectrometry experiment. Also provided are specific biomarkers of bladder cancer. The methods for isolating and analysing sialylated proteins can also by applied to phosphorylated proteins.
REFERENCES:
patent: 2007/0227974 (2007-10-01), Anders et al.
patent: 2008/0261321 (2008-10-01), Patton et al.
patent: 1 428 878 (2004-06-01), None
patent: 1 477 800 (2004-11-01), None
Stewart et al “18O Labeling:a tool for proteomics” Pub 2001 Rapid Commun. Mass Spectrom 15 pp. 2456-2465.
Mirgorodskaya et al “Quantitation of peptides and proteins by matrix-assisted laser desorption /ionization mass spectrometry using 18O-labeled internal standards” Pub 2000 Rapid ommuications in Mass Spectrometry 14 pp. 1226-1232.
Aparna H S et al , Isolation and Characterization of a Sialo-Glycopeptide from Buffalo Colostrum, Glycoconjugate Journal (1996) vol. 13: pp. 63-67, 1996 by Chapman & Hall.
Connor P A et al , Phosphate Adsorption onto TiO2 from Aqueous Solutions: An in Situ Internal Reflection Infrared Spectroscopic Study, Langmuir 1999, vol. 15, pp. 2916-2921, 1999 American Chemical Society, published on web Mar. 27, 1999.
Dobson K D et al., In Situ Infrared Spectroscopic Analysis of the Adsorption of Aromatic Carboxylic Acids to TiO2, ZrO2, A12O3, and Ta2O5 from Aqueous Solutions, Spectrochimica Acta Part A vol. 56 (2000), pp. 557-565. 2000 by Elsevier Science B V , New Zealand.
Ficarro S et al , Phosphoproteome Analysis of Capacitated Human Sperm, The Journal of Biological Chemistry, vol. 278, No. 13, Issue of Mar. 28, pp. 11579-11589, 2003, by JBC Papers in Press, Dec. 30, 2002, Printed in U S A.
Figeys D et al , Electrophoresis Combined With Novel Mass Spectrometry Techniques: Powerful Tools for the Analysis of Proteins and Proteomes, Electrophoresis 1998, vol. 19. pp. 1811-1818, 1998 by Wiley-VCH Verlag GmbH, 69451 Weinheim.
Gruhler A et al , Quantitative Phosphoproteomics Applied to the Yeast Pheromone Signaling Pathway, Molecular & Cellular Proteomics 4 3, pp. 310-327, 2005 by The American Society for Biochemistry and Molecular Biology, Inc.
Irmak S et al., Stage-dependent Increase of Orosomucoid and Zinc-Alpha2-Glycoprotein in Urinary Bladder Cancer, Proteomics 2005, vol. 5, pp. 4296-4304, 2005 by Wiley-VCH Verlag GmbH & Co KGaA Weinheim.
Kweon H K et al , Selective Zirconium Dioxide-Based Enrichment of Phosphorylated Peptides for Mass Spectrometric Analysis, Analytical Chemistry, vol. 78, No. 6, Mar. 15, 2006, pp. 1743-1749.
Larsen M R et al , Highly Selective Enrichment of Phosphorylated Peptides from Peptide Mixtures Using Titanium Dioxide Microcolumns, Molecular & Cellular Proteomics 4 7, pp. 873-886, 2005 by the American Society for Biochemistry and Molecular Biology, Inc.
Li S et al , Iron(III)-Immobilized Metal Ion Affinity Chromatography and Mass Spectrometry for the Purification and Characterization of Synthetic Phosphopeptides, Analytical Biochemistry vol. 270, pp. 9-14 (1999), Article ID abio 1999 4060 , 1999 by Academic Press.
Neville DCA et al , Evidence for Phosphorylation of Serine 753 in CFTR Using a Novel Metal-Ion Affinity Resin and Matrix-assisted Laser Desorption Mass Spectrometry, Protein Science 1997 vol. 6, pp. 2436-2445, 1997 by Cold Spring Harbor Laboratory Press.
Nühse T S et al , Large-scale Analysis of in Vivo Phosphorylated Membrane Proteins by Immobilized Metal Ion Affinity Chromatography and Mass Spectrometry, Molecular & Cellular Proteomics vol. 2 11, pp. 1234-1243, 2003 by American Society for Biochemistry and Molecular Biology, Inc.
Pal S et al , Purification and Characterization of 9-O-acetylated Siaioglycoproteins from Leukemic Cells and Their Potential as Immunological Tool for Monitoring Childhood Acute Lymphoblastic Leukemia, Glycobiology vol. 14, No. 10, 2004, pp. 859-870, by Advance Access Publication on Jun. 9, 2004.
Pinkse M W H et al , Selective Isolation at the Femtomole Level of Phosphopeptides from Proteolytic Digests Using 2D-NanoLC-ESI-MS/MS and Titanium Oxide Precolumns. Analytical Chemistry, vol. 76, No. 14, Jul. 15, 2004, pp. 3935-3943, 2004 by American Chemical Society.
Posewitz M C et al., Immobilized Gallium(III) Affinity Chromatography of Phosphopeptides, Analytical Chemistry, vol. 71, No. 14, Jul. 15, 1999, pp. 2883-2892, 1999 by American Chemical Society.
Rinal Ducci S. et al , Novel Protein Phosphorylation Site Identification in Spinach Stroma Membranes by Titanium Dioxide Microcolumns and Tandem Mass Spectrometry, Journal of Proteome Research 2006, vol. 5, pp. 973-982, 2006 by American Chemical Society.
Treuheit M J et al , Analysis of the Five Glycosylation Sites of Human Alpha1-acid Glycoprotein, Biochem J (1992), vol. 283, pp. 105-112, Printed in Great Britain.
Birch & Stewart Kolasch & Birch, LLP
Lilling Herbert J.
Syddansk Universitet
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