Methods for isolating recombinant .beta.-casein

Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues – Casein or caseinate

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530361, 530412, 530418, 530427, 435 691, 435 711, 435 712, C07K 1452, C12P 2106

Patent

active

061214213

ABSTRACT:
The invention provides a method for isolating a protein, specifically .beta.-casein, recombinantly produced in cells that have been genetically engineered to produce the protein. The method involves forming a paste, homogenate or lysate of the cells following fermentation to produce the protein, and performing a two-stage extraction procedure to isolate of the protein. By this method, recombinantly produces .beta.-casein can be isolated with less complexity and greater efficiency than in previously known methods for its isolation.

REFERENCES:
patent: 5466781 (1995-11-01), Dorin et al.
Hansson et al., (Protein Expression and Purification, vol. 4, pp. 373-381, 1993.
Simons et al, Protein Engineering, vol. 6 No. 7, pp. 763-770, 1993.
Bonnerjea et al, Bio/Technology, vol. 4, pp. 954-958, Nov. 1986.
Burgess et al (Acc. Symup. Ser. 578 C Structure and Flow in Surf. Sol.) pp. 380-393, 1994.

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