Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Reexamination Certificate
2005-09-13
2005-09-13
Guzo, David (Department: 1636)
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
C435S069100, C435S192000, C536S023200, C536S023700
Reexamination Certificate
active
06942973
ABSTRACT:
The present invention relates to methods for isolating a gene encoding an enzyme, comprising: (a) adding a mixture of labeled first nucleic acid probes from a microbial strain cultured on medium without the substrate, and labeled second nucleic acid probes from a microbial strain cultured on medium with the substrate, to an array of random nucleic acid fragments of the microbial strain where the labeled nucleic acids hybridize to complementary sequences of the genomic fragments in the array, wherein the first nucleic acid probes are labeled with a first reporter and the second nucleic acid probes are labeled with a second reporter; (b) examining the array under conditions wherein the relative expression of the genes of the microbial strain is determined by the observed hybridization reporter signal of each spot in the array; and (c) isolating a gene from the microbial strain that encodes an enzyme that degrades the substrate. The present invention also relates to isolated genes obtained by such methods.
REFERENCES:
patent: 2002/0146721 (2002-10-01), Berka et al.
Whitman et al., Expression of fungal MN peroxidase inE. Coliand refolding to yield active enzyme, 1995, Biochemical and Biophtsical Research Communications, vol. 216, pp. 1013-1017.
Mayfield et al., Characterization of the mnp2 gene encoding manganese peroxidase isozyme 2 from the basidiomycetephanerochaete chrysosporium, 1994, GENE, vol. 142, pp. 213-235.
Schena, Genome analysis with gene expression microassays, 1996, BIOESSAYS, vol. 18, pp. 427-431.
Brzostowica et al., Simultaneous identification of two cyclohexanone oxidation genes from an environmentalbrevibacteriumisolate using mRNA differential display, 2000, Journal of Bacteriology, vol. 182, pp. 4241-4248.
Watson et al., Gene chips and array revealed: A primer on their power and their uses, 1999, Biological Psychiatry, vol. 45, pp. 533-543.
DeRisi et al., Exploring the metabolic and genetic control of gene expression on a genomic scale, 1997, SCIENCE, vol. 278, pp. 680-686.
Berka Randy M.
Yaver Debbie S.
Guzo David
Novozymes Biotech Inc.
Starnes, Esq. Robert L.
Sullivan Daniel M.
LandOfFree
Methods for isolating genes from microorganisms does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Methods for isolating genes from microorganisms, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Methods for isolating genes from microorganisms will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-3376166