Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Hydrolase
Patent
1992-04-03
1994-07-12
Naff, David M.
Chemistry: molecular biology and microbiology
Enzyme , proenzyme; compositions thereof; process for...
Hydrolase
4352567, 435945, C12N 942, C12N 118, C12N 100
Patent
active
053288416
ABSTRACT:
Methods for isolating EG III cellulase component and enriched EG III cellulase in polyethylene glycol are disclosed. The methods comprise adding an inorganic salt and polyethylene glycol having a molecular weight of from about 5,000 to 10,000 to an aqueous mixture of cellulase proteins under conditions to create a two-phase system. Next, the cellulase proteins other than EG III are separated in an EG III-poor aqueous phase while EG III cellulase component is retained in an EG III-rich polyethylene glycol phase. Lastly, the EG III component can be separated from the polyethylene glycol. The preferred method of separation is alcohol precipitation.
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Bower Benjamin S.
Clarkson Kathleen A.
Larenas Edmund
Lorch Jeffrey D.
Weiss Geoffrey L.
Genencor International Inc.
Krupen Karen I.
Meller Michael V.
Naff David M.
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