Methods for introducing a fertility restorer gene and for produc

Multicellular living organisms and unmodified parts thereof and – Method of introducing a polynucleotide molecule into or... – The polynucleotide alters fat – fatty oil – ester-type wax – or...

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800200, 800205, 800250, 800255, 800DIG15, 800DIG17, 4351722, 47 58, A01H 500, A01H 102, C12N 514, C12N 1502

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056440669

ABSTRACT:
A method is provided for introducing a fertility restorer gene of a Raphanus plant into a Brassica plant by means of cell fusion or intergeneric cross and a Brassica plant produced thereby. Also provided is a method for producing an F.sub.1 hybrid of a rapeseed plant utilizing Kosena-radish-derived CMS gene and a fertility restorer gene derived from any of the radish plants and an F.sub.1 hybrid of a rapeseed plant produced thereby.

REFERENCES:
Genome, R. Pellan-Delourme et al., "Cytoplasmic Male Sterility in Rapessed (Brassica napus L.): Female Fertility of Restored Rapeseed with Ogura and Cybrids Cytoplasms", vol. 30, 1988, pp. 234-238.
Mol. Gen. Genet., G. Pelletier et al., "Intergeneric Cytoplasmic Hybridization in Cruciferae by Protoplast Fusion", (1983) 191:244-250.
Paulmann et al., Plant Breeding, "Effective Transfer of Cytoplasmic Male Sterility from Radish (Raphanus sativus L.) to Rape (Brassica napus L.).sup.1)", vol. 100, pp. 299-309 (1988).
Bajaj, Biotechnology in Agriculture and Forestry, vol. 10, "Legumes and Oilseed Crops I", Ch. IV. 3, pp. 418-433 (1990).
Poehlman, Breeding field Crops, Ch. 7, "Fertility Regulating Mechanisms and Their Manipulation", pp. 129-147 (1986).
Pelletier et al., Molecular and General Genetics, "Intergeneric Cytoplasmic Hybridization in Cruciferae by Protoplast Fusion", vol. 191, pp. 244-250 (1983).
Harbred et al. 1969. Euphytica. 18;425-429.
Heyn. 1978. Proceedings 5th International Rapeseed Conference vol. 1:82-83.
Dolstra. 1982. Synthesis and fertility of x Brassicoraphanus and Ways of Transferring Raphanus characters to Brassica. pp. 1-90. Agricultural Research Reports. Wageningen.

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