Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Patent
1995-06-06
1996-12-10
Guzo, David
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
435 72, 4351721, 4351723, 530327, 530328, G01N 3353
Patent
active
055829996
ABSTRACT:
Novel compositions and methods that result in the inactivation of IFN.gamma. inducible transcription factors by binding to the transcription factor in the cell and inhibiting the transcriptional activity of the factors are disclosed. The composition comprises an isolated peptide that contains the amino acid sequence Xaa.sub.1 -Asp-Xaa.sub.2 -Xaa.sub.3 -His SEQ ID No. 1 where Xaa.sub.1 is phosphorylated tyrosine and Xaa.sub.2 and Xaa.sub.3 are any amino acid, or derivatives and functional equivalents thereof, that is capable of specifically binding to the p91 family of IFN.gamma. inducible transcription factors in a cell. A method for inhibiting the intracellular activation of a transcription factor by introducing into a cell an effective amount of a peptide that contains the 5 amino acid sequence described or a derivative thereof, which specifically binds to a transcription factor in the cell is also provided.
REFERENCES:
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Schindler et al., (1992), Proteins of transcription factor ISGF-3: One gene encodes the 91-and 84-kDa ISGF-3 proteins that are activated by interferon .alpha., Proc. Natl. Acad. Sci. 89:7836-9.
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Farrar Michael A.
Greenlund Andrew C.
Schreiber Robert D.
Guzo David
Washington University
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